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. 1993 Aug 25;21(17):4143–4146. doi: 10.1093/nar/21.17.4143

A new PCR based method for the generation of nested deletions.

J M Whitcomb 1, A Rashtchian 1, S H Hughes 1
PMCID: PMC310022  PMID: 7690475

Abstract

We have developed a simple, PCR-based protocol, random primed/anchored-PCR (RPA-PCR), that allows the selective amplification and efficient cloning of segments that are adjacent to any known sequence. We demonstrate that RPA-PCR can be used to prepare a nested set of evenly spaced deletions suitable for DNA sequencing. However, it should also be possible to use this technique for a number of other purposes: generating deletions for the analysis of eukaryotic promoters, extending cDNA clones in the 5' direction, cloning the insertion sites of retroviral proviruses and transposons, and analyzing intron/exon boundaries.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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