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. Author manuscript; available in PMC: 2012 Jun 1.
Published in final edited form as: Laryngoscope. 2011 Apr 7;121(6):1313–1319. doi: 10.1002/lary.21798

Figure 4. Resveratrol does not Increase Cellular cAMP or Phosphorylate the CFTR R-Domain.

Figure 4

Figure 4

(A) Cellular cAMP was minimal in the presence of resveratrol and not significantly changed when compared to DMSO controls. The adenylate cyclase agonist forskolin was used as a positive control. (B) Polyclonal NIH-3T3 cells expressing HA-tagged R-domain were treated with resveratrol for 20 minutes, and compared to forskolin (100 nM) or DMSO control in an assay of R-D phosphorylation. Phosphorylation results in a 2-4 kD shift in migration (white arrow).