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. 2011 Apr 26;50(21):4393–4395. doi: 10.1021/bi2004813

Figure 1.

Figure 1

Effect of benzbromarone on maturation of CFTR and P-glycoprotein processing mutants. (A) Immunoblot analysis of cells expressing CFTR mutant ΔF508 or H1085R or the P-gp G251V processing mutant after treatment with the indicated concentrations of benzbromarone (Benz) for 40 h. (B) Iodide efflux assays performed on BHK cells stably expressing wild-type, ΔF508, or H1085R CFTR. Cells expressing mutant CFTRs were assayed after treatment with 0.05 mM benzbromarone for 40 h. (C) Effect of benzbromarone on cross-linking (X-link) between cysteines in TMD1 and TMD2 (M348C/T1142C) or NBD1 and TMD2 (V510C/A1067C).(7) (D) Immunoblot of cells expressing CFTR TMD1+2 in the absence (−) or presence (+) of 0.05 mM benzbromarone. Samples were treated with (+) or without (−) endoglycosidase H (Endo H).