Skip to main content
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1993 Sep 11;21(18):4418–4419. doi: 10.1093/nar/21.18.4418

Simultaneous randomization of antibody CDRs by a synthetic ligase chain reaction strategy.

S J Deng 1, C R MacKenzie 1, S A Narang 1
PMCID: PMC310099  PMID: 8415016

Abstract

Full text

PDF

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anand N. N., Mandal S., MacKenzie C. R., Sadowska J., Sigurskjold B., Young N. M., Bundle D. R., Narang S. A. Bacterial expression and secretion of various single-chain Fv genes encoding proteins specific for a Salmonella serotype B O-antigen. J Biol Chem. 1991 Nov 15;266(32):21874–21879. [PubMed] [Google Scholar]
  2. Barany F. Genetic disease detection and DNA amplification using cloned thermostable ligase. Proc Natl Acad Sci U S A. 1991 Jan 1;88(1):189–193. doi: 10.1073/pnas.88.1.189. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Barbas C. F., 3rd, Bain J. D., Hoekstra D. M., Lerner R. A. Semisynthetic combinatorial antibody libraries: a chemical solution to the diversity problem. Proc Natl Acad Sci U S A. 1992 May 15;89(10):4457–4461. doi: 10.1073/pnas.89.10.4457. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hoogenboom H. R., Winter G. By-passing immunisation. Human antibodies from synthetic repertoires of germline VH gene segments rearranged in vitro. J Mol Biol. 1992 Sep 20;227(2):381–388. doi: 10.1016/0022-2836(92)90894-p. [DOI] [PubMed] [Google Scholar]
  5. Landegren U., Kaiser R., Sanders J., Hood L. A ligase-mediated gene detection technique. Science. 1988 Aug 26;241(4869):1077–1080. doi: 10.1126/science.3413476. [DOI] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES