Skip to main content
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1993 Sep 11;21(18):4424–4425. doi: 10.1093/nar/21.18.4424

Addition of a class IIS enzyme site in the mutagenic primer to improve two-step PCR-based targeted mutagenesis.

J Armengaud 1, Y Jouanneau 1
PMCID: PMC310102  PMID: 8415019

Abstract

Full text

PDF
4424

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bolton B. J., Schmitz G. G., Jarsch M., Comer M. J., Kessler C. Ksp632I, a novel class-IIS restriction endonuclease from Kluyvera sp. strain 632 with the asymmetric hexanucleotide recognition sequence: 5'-CTCTTC(N)1-3' 3'-GAGAAG(N)4-5'. Gene. 1988 Jun 15;66(1):31–43. doi: 10.1016/0378-1119(88)90222-3. [DOI] [PubMed] [Google Scholar]
  2. Duport C., Jouanneau Y., Vignais P. M. Nucleotide sequence of fdxA encoding a 7Fe ferredoxin of Rhodobacter capsulatus. Nucleic Acids Res. 1990 Aug 11;18(15):4618–4618. doi: 10.1093/nar/18.15.4618. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Kuipers O. P., Boot H. J., de Vos W. M. Improved site-directed mutagenesis method using PCR. Nucleic Acids Res. 1991 Aug 25;19(16):4558–4558. doi: 10.1093/nar/19.16.4558. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Landt O., Grunert H. P., Hahn U. A general method for rapid site-directed mutagenesis using the polymerase chain reaction. Gene. 1990 Nov 30;96(1):125–128. doi: 10.1016/0378-1119(90)90351-q. [DOI] [PubMed] [Google Scholar]
  5. Mole S. E., Iggo R. D., Lane D. P. Using the polymerase chain reaction to modify expression plasmids for epitope mapping. Nucleic Acids Res. 1989 Apr 25;17(8):3319–3319. doi: 10.1093/nar/17.8.3319. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Sharrocks A. D., Shaw P. E. Improved primer design for PCR-based, site-directed mutagenesis. Nucleic Acids Res. 1992 Mar 11;20(5):1147–1147. doi: 10.1093/nar/20.5.1147. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES