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. 1994 Dec 25;22(25):5600–5607. doi: 10.1093/nar/22.25.5600

Trinucleotide phosphoramidites: ideal reagents for the synthesis of mixed oligonucleotides for random mutagenesis.

B Virnekäs 1, L Ge 1, A Plückthun 1, K C Schneider 1, G Wellnhofer 1, S E Moroney 1
PMCID: PMC310122  PMID: 7838712

Abstract

Trinucleotide phosphoramidites representing codons for all 20 amino acids have been prepared and used in automated, solid-phase DNA synthesis. In contrast to an earlier report, we show that these substances can be used to introduce entire codons into oligonucleotides in excess of 98% yield, and are ideal reagents for the synthesis of mixed oligonucleotides for random mutagenesis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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