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. 1992 Jan 25;20(2):257–261. doi: 10.1093/nar/20.2.257

Promoter selectivity of the stationary-phase forms of Escherichia coli RNA polymerase and conversion in vitro of the S1 form enzyme into a log-phase enzyme-like form.

M Ozaki 1, N Fujita 1, A Wada 1, A Ishihama 1
PMCID: PMC310363  PMID: 1741250

Abstract

Upon growth transition of Escherichia coli cells from exponential to stationary phase, RNA polymerase is converted into at least three different forms (S1, S2 and S3), which could be separately isolated by phosphocellulose column chromatography (Ozaki et al., 1991 (2)). Here, the promoter selectivity of these three stationary-phase enzymes was examined using an in vitro mixed transcription system and an E. coli promoter collection. These altered forms of RNA polymerase showed different recognition properties of promoters from that by the log-phase holoenzyme (L1). One of the stationary-phase RNA polymerases, S1, was found to be converted in vitro into an enzyme like the log-phase form following incubation with nucleotides or pyrophosphate. The conversion was indicated by not only the shift of elution position from a phosphocellulose column but also the change in the promoter selectivity. These results may suggest that RNA polymerase is interconvertible between different forms with different promoter selectivity by interaction with a phosphorylated compound(s).

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aldea M., Garrido T., Hernández-Chico C., Vicente M., Kushner S. R. Induction of a growth-phase-dependent promoter triggers transcription of bolA, an Escherichia coli morphogene. EMBO J. 1989 Dec 1;8(12):3923–3931. doi: 10.1002/j.1460-2075.1989.tb08573.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Atlung T., Nielsen A., Hansen F. G. Isolation, characterization, and nucleotide sequence of appY, a regulatory gene for growth-phase-dependent gene expression in Escherichia coli. J Bacteriol. 1989 Mar;171(3):1683–1691. doi: 10.1128/jb.171.3.1683-1691.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Chao L., Speyer J. F. A new form of RNA polymerase isolated from Escherichia coli. Biochem Biophys Res Commun. 1973 Mar 17;51(2):399–405. doi: 10.1016/0006-291x(73)91271-0. [DOI] [PubMed] [Google Scholar]
  4. Erickson J. W., Vaughn V., Walter W. A., Neidhardt F. C., Gross C. A. Regulation of the promoters and transcripts of rpoH, the Escherichia coli heat shock regulatory gene. Genes Dev. 1987 Jul;1(5):419–432. doi: 10.1101/gad.1.5.419. [DOI] [PubMed] [Google Scholar]
  5. Fujita N., Nomura T., Ishihama A. Promoter selectivity of Escherichia coli RNA polymerase. Purification and properties of holoenzyme containing the heat-shock sigma subunit. J Biol Chem. 1987 Feb 5;262(4):1855–1859. [PubMed] [Google Scholar]
  6. Fukuda R., Iwakura Y., Ishihama A. Heterogeneity of RNA polymerase in Escherichia coli. I. A new holoenzyme containing a new sigma factor. J Mol Biol. 1974 Mar;83(3):353–367. doi: 10.1016/0022-2836(74)90284-8. [DOI] [PubMed] [Google Scholar]
  7. Helmann J. D., Chamberlin M. J. Structure and function of bacterial sigma factors. Annu Rev Biochem. 1988;57:839–872. doi: 10.1146/annurev.bi.57.070188.004203. [DOI] [PubMed] [Google Scholar]
  8. Hernández-Chico C., San Millán J. L., Kolter R., Moreno F. Growth phase and ompR regulation of transcription of microcin B17 genes. J Bacteriol. 1986 Sep;167(3):1058–1065. doi: 10.1128/jb.167.3.1058-1065.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Ishihama A. Promoter selectivity of prokaryotic RNA polymerases. Trends Genet. 1988 Oct;4(10):282–286. doi: 10.1016/0168-9525(88)90170-9. [DOI] [PubMed] [Google Scholar]
  10. Izui K., Miwa T., Kajitani M., Fujita N., Sabe H., Ishihama A., Katsuki H. Promoter analysis of the phosphoenolpyruvate carboxylase gene of Escherichia coli. Nucleic Acids Res. 1985 Jan 11;13(1):59–71. doi: 10.1093/nar/13.1.59. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. KORNBERG A., KORNBERG S. R., SIMMS E. S. Metaphosphate synthesis by an enzyme from Escherichia coli. Biochim Biophys Acta. 1956 Apr;20(1):215–227. doi: 10.1016/0006-3002(56)90280-3. [DOI] [PubMed] [Google Scholar]
  12. Kajitani M., Ishihama A. Determination of the promoter strength in the mixed transcription system: promoters of lactose, tryptophan and ribosomal protein L10 operons from Escherichia coli. Nucleic Acids Res. 1983 Feb 11;11(3):671–686. doi: 10.1093/nar/11.3.671. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Kubota M., Yamazaki Y., Ishihama A. Random screening of promoters from Escherichia coli and classification based on the promoter strength. Jpn J Genet. 1991 Aug;66(4):399–409. doi: 10.1266/jjg.66.399. [DOI] [PubMed] [Google Scholar]
  14. Lange R., Hengge-Aronis R. Identification of a central regulator of stationary-phase gene expression in Escherichia coli. Mol Microbiol. 1991 Jan;5(1):49–59. doi: 10.1111/j.1365-2958.1991.tb01825.x. [DOI] [PubMed] [Google Scholar]
  15. MUDD S., YOSHIDA A., KOIKE M. Polyphosphate as accumulator of phosphorus and energy. J Bacteriol. 1958 Feb;75(2):224–235. doi: 10.1128/jb.75.2.224-235.1958. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Nomura T., Fujita N., Ishihama A. Expression of the leuX gene in Escherichia coli. Regulation at transcription and tRNA processing steps. J Mol Biol. 1987 Oct 20;197(4):659–670. doi: 10.1016/0022-2836(87)90472-4. [DOI] [PubMed] [Google Scholar]
  17. Ueshima R., Fujita N., Ishihama A. DNA supercoiling and temperature shift affect the promoter activity of the Escherichia coli rpoH gene encoding the heat-shock sigma subunit of RNA polymerase. Mol Gen Genet. 1989 Jan;215(2):185–189. doi: 10.1007/BF00339716. [DOI] [PubMed] [Google Scholar]
  18. Wada A., Yamazaki Y., Fujita N., Ishihama A. Structure and probable genetic location of a "ribosome modulation factor" associated with 100S ribosomes in stationary-phase Escherichia coli cells. Proc Natl Acad Sci U S A. 1990 Apr;87(7):2657–2661. doi: 10.1073/pnas.87.7.2657. [DOI] [PMC free article] [PubMed] [Google Scholar]

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