TABLE 2 .
Fatty acid composition of the wild-type (M1-WT) and mutant (M1-SDHHBtail) GAS strainsa
| Fatty acid peak | Fatty acid compositionb
|
P value | |
|---|---|---|---|
| M1-WT | M1-SDHHBtail | ||
| 12:0 | 1.94 ± 0.41 | 5.04 ± 0.46 | 0.0016 |
| 13:0 | 1.10 ± 0.21 | 1.37 ± 0.17 | 0.0926 |
| 14:0 | 3.66 ± 0.79 | 8.41 ± 0.24 | 0.0050 |
| 16:1 w9cc | 13.53 ± 1.77 | 19.18 ± 4.53 | 0.0917 |
| 16:1 w7c | 21.21 ± 4.14 | 31.27 ± 7.04 | 0.0613 |
| 16:1 w5c | 3.18 ± 0.39 | 4.29 ± 0.92 | 0.0978 |
| 16:0 | 71.58 ± 9.37 | 109.79 ± 9.54 | 0.0078 |
| 18:1 w9c | 20.9 ± 1.07 | 29.19 ± 3.02 | 0.0233 |
| 18:1 w7c | 66.88 ± 1.16 | 74.76 ± 9.52 | 0.1455 |
| 18:1 w5c | 4.35 ± 0.12 | 4.82 ± 0.51 | 0.1300 |
| 18:0 | 18.23 ± 1.54 | 38.49 ± 7.37 | 0.0215 |
| 19:1 iso I | 1.82 ± 0.21 | 2.92 ± 0.24 | 0.0049 |
| Total fatty acid | 228.71 ± 19.5 | 330.69 ± 26.6 | 0.0064 |
| % fatty acid in cell mass | 0.47 + 0.06 | 0.77 ± 0.10 | 0.0129 |
a
The rows with significant differences as evaluated by one-tailed nonparametric t test with Welch’s correction are shaded.
b
Mean ± SD of three independent experiments. Shaded rows highlight significant increase in specific fatty acid contents.
c
16:1 w9c, fatty acid with 16-carbon chain with one double bond located at the ninth carbon from the ω (omega) end of the chain.