Skip to main content
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1993 Dec 25;21(25):6047–6048. doi: 10.1093/nar/21.25.6047

Determination of plasmid copy number in yeast transformants by means of agarose plugs.

A M Guerrini 1, F Ascenzioni 1, T Gallo 1, P Donini 1
PMCID: PMC310495  PMID: 8290370

Abstract

Full text

PDF
6047

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Beggs J. D. Transformation of yeast by a replicating hybrid plasmid. Nature. 1978 Sep 14;275(5676):104–109. doi: 10.1038/275104a0. [DOI] [PubMed] [Google Scholar]
  2. Botstein D., Falco S. C., Stewart S. E., Brennan M., Scherer S., Stinchcomb D. T., Struhl K., Davis R. W. Sterile host yeasts (SHY): a eukaryotic system of biological containment for recombinant DNA experiments. Gene. 1979 Dec;8(1):17–24. doi: 10.1016/0378-1119(79)90004-0. [DOI] [PubMed] [Google Scholar]
  3. Cryer D. R., Eccleshall R., Marmur J. Isolation of yeast DNA. Methods Cell Biol. 1975;12:39–44. doi: 10.1016/s0091-679x(08)60950-4. [DOI] [PubMed] [Google Scholar]
  4. Feinberg A. P., Vogelstein B. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem. 1983 Jul 1;132(1):6–13. doi: 10.1016/0003-2697(83)90418-9. [DOI] [PubMed] [Google Scholar]
  5. Guerrini A. M., Boglione C., Ascenzioni F., Donini P. On the level of plasmid-bearing cells in transformed cultures of Saccharomyces cerevisiae. Yeast. 1991 Dec;7(9):943–952. doi: 10.1002/yea.320070906. [DOI] [PubMed] [Google Scholar]
  6. Szostak J. W., Blackburn E. H. Cloning yeast telomeres on linear plasmid vectors. Cell. 1982 May;29(1):245–255. doi: 10.1016/0092-8674(82)90109-x. [DOI] [PubMed] [Google Scholar]
  7. Tsao S. G., Brunk C. F., Pearlman R. E. Hybridization of nucleic acids directly in agarose gels. Anal Biochem. 1983 Jun;131(2):365–372. doi: 10.1016/0003-2697(83)90185-9. [DOI] [PubMed] [Google Scholar]
  8. Wellinger R. J., Zakian V. A. Lack of positional requirements for autonomously replicating sequence elements on artificial yeast chromosomes. Proc Natl Acad Sci U S A. 1989 Feb;86(3):973–977. doi: 10.1073/pnas.86.3.973. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES