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. 1993 Dec 11;21(24):5595–5599. doi: 10.1093/nar/21.24.5595

Structure specific ds/ss-RNase activity in the extreme halophile Halobacterium salinarium.

P Stolt 1, W Zillig 1
PMCID: PMC310521  PMID: 7506828

Abstract

A ds/ss-RNA processing activity involved in antisense-RNA mediated gene regulation in the extremely halophilic archaebacterium Halobacterium salinarium was investigated in vivo. H.salinarium cells were transformed with DNA encoding an RNA species complementary to a part of the major lytic transcript, termed T4, of the H.salinarium phage phi H. The transformants transcribing this construct, when infected by phage were able to process T4 in a similar way to the processing of the lytic transcript denoted T1, in the natural sense-antisense system. Processing of T4 was not observed under normal phage growth on wild-type cells. Thus the antisense-RNA mediated processing activity earlier reported is dependent on the presence of an RNA duplex and is not sequence specific.

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Selected References

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