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. 1993 Dec 11;21(24):5600–5603. doi: 10.1093/nar/21.24.5600

Activity of hammerhead ribozymes containing non-nucleotidic linkers.

J B Thomson 1, T Tuschl 1, F Eckstein 1
PMCID: PMC310522  PMID: 7506829

Abstract

Hammerhead ribozymes were synthesized in which the tetranucleotide loop II was replaced by non-nucleotidic linkers of 7, 13, 17 and 19 atoms length. Ribozymes with 17 and 19 atom linkers, in combination with a 4 base pair stem II, had catalytic efficiencies which were 2 fold increased to that of the parent ribozyme with a tetranucleotide loop. Ribozymes with these linkers, but in combination with a 2 base pair stem II, showed a 2 fold decrease in catalytic efficiency when compared to the parent ribozyme. Prolonged preincubation in the presence of MgCl2 was required for hexaethylene glycol linker-modified ribozymes to obtain maximum activity and reproducible kinetic data.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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