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. 1993 Nov 25;21(23):5403–5407. doi: 10.1093/nar/21.23.5403

Oligonucleotide circularization by template-directed chemical ligation.

N G Dolinnaya 1, M Blumenfeld 1, I N Merenkova 1, T S Oretskaya 1, N F Krynetskaya 1, M G Ivanovskaya 1, M Vasseur 1, Z A Shabarova 1
PMCID: PMC310578  PMID: 8265356

Abstract

An efficient method for producing the covalent closure of oligonucleotides on complementary templates by the action of BrCN was developed. A rational design of linear precursor oligonucleotides was studied, and the effect of factors such as oligonucleotide concentration and oligomer-template length ratio was evaluated. The efficiency of circularization was shown to correlate well with the secondary structure of the precursor oligomer (as predicted by a simple computer analysis), hairpin-like structures bearing free termini clearly favouring the circularization reaction. A novel idea, consisting of the incorporation of non-nucleotide insertions in the precursor oligomer (namely, 1,2-dideoxy-D-ribofuranose residues), may render this method universal and highly effective. An original set of assays was developed to confirm the circular structure of the covalently closed oligonucleotides.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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