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. 1993 Nov 11;21(22):5283–5284. doi: 10.1093/nar/21.22.5283

PCR amplification of highly GC-rich DNA template after denaturation by NaOH.

R K Agarwal 1, A Perl 1
PMCID: PMC310653  PMID: 8255790

Abstract

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Banki K., Maceda J., Hurley E., Ablonczy E., Mattson D. H., Szegedy L., Hung C., Perl A. Human T-cell lymphotropic virus (HTLV)-related endogenous sequence, HRES-1, encodes a 28-kDa protein: a possible autoantigen for HTLV-I gag-reactive autoantibodies. Proc Natl Acad Sci U S A. 1992 Mar 1;89(5):1939–1943. doi: 10.1073/pnas.89.5.1939. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Dutton C. M., Paynton C., Sommer S. S. General method for amplifying regions of very high G+C content. Nucleic Acids Res. 1993 Jun 25;21(12):2953–2954. doi: 10.1093/nar/21.12.2953. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Erlich H. A., Gelfand D., Sninsky J. J. Recent advances in the polymerase chain reaction. Science. 1991 Jun 21;252(5013):1643–1651. doi: 10.1126/science.2047872. [DOI] [PubMed] [Google Scholar]
  4. Perl A., Rosenblatt J. D., Chen I. S., DiVincenzo J. P., Bever R., Poiesz B. J., Abraham G. N. Detection and cloning of new HTLV-related endogenous sequences in man. Nucleic Acids Res. 1989 Sep 12;17(17):6841–6854. doi: 10.1093/nar/17.17.6841. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Sarkar G., Kapelner S., Sommer S. S. Formamide can dramatically improve the specificity of PCR. Nucleic Acids Res. 1990 Dec 25;18(24):7465–7465. doi: 10.1093/nar/18.24.7465. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Shimotohno K., Takahashi Y., Shimizu N., Gojobori T., Golde D. W., Chen I. S., Miwa M., Sugimura T. Complete nucleotide sequence of an infectious clone of human T-cell leukemia virus type II: an open reading frame for the protease gene. Proc Natl Acad Sci U S A. 1985 May;82(10):3101–3105. doi: 10.1073/pnas.82.10.3101. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Srivastava B. I., Banki K., Perl A. Human T-cell leukemia virus type I or a related retrovirus in patients with mycosis fungoides/Sézary syndrome and Kaposi's sarcoma. Cancer Res. 1992 Aug 15;52(16):4391–4395. [PubMed] [Google Scholar]

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