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. 2011 Jul 1;138(13):2693–2703. doi: 10.1242/dev.063412

Fig. 2.

Fig. 2.

Loss of Activin signaling in the PG leads to a failure to initiate metamorphosis. (A) Knockdown of dSmad2 (left panel) results in larval developmental arrest and giant larvae compared with control (right panel). (B) Immunostaining for Coracle in both the control and dSmad2 RNAi larvae. DAPI is in blue. (C,C′) DAPI staining of RGBCs of the control and dSmad2 RNAi larvae. (D,D′) DAPI staining of wing imaginal discs of the control and dSmad2 RNAi larvae. (E) The whole larva ecdysteroid titer of the control and dSmad2 RNAi larvae. Data are from a single determination using 50 larvae for each data point. (F) 20E feeding at 48 hours after the second ecdysis rescues the dSmad2 RNAi larvae to the pupa stage.