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Journal of Visualized Experiments : JoVE logoLink to Journal of Visualized Experiments : JoVE
. 2008 Aug 27;(18):891. doi: 10.3791/891

Preparation and Fractionation of Xenopus laevis Egg Extracts

Marie K Cross 1, Maureen Powers 1
PMCID: PMC3110484  PMID: 19066500

Abstract

Crude and fractionated Xenopus egg extracts can be used to provide ingredients for reconstituting cellular processes for morphological and biochemical analysis. Egg lysis and differential centrifugation are used to prepare the crude extract which in turn in used to prepare fractionated extracts and light membrane preparations.


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Protocol

The complete text protocol for this experimental approach is available in Current Protocols in Cell Biology.


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