Figure 3.

Discrimination between the fully complementary hybrid (closed triangles) and the 2-bp mismatched hybrid (open triangles) by electric field-induced denaturation on the same surface. After passive hybridization for 14 h, a dc electrochemical potential of −300 mV vs. Ag/AgCl was applied and the percent hybridization monitored as loss of ssDNA from the interface by in situ SPR. The same discrimination is also observed for hybrids formed by electrostatically assisted hybridization. Experimental conditions were 1 M NaCl solution containing 1 μM oligonucleotide target. Data were obtained by using the same regenerated probe surface.