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. 1993 Sep 25;21(19):4586–4591. doi: 10.1093/nar/21.19.4586

Cloning the RAD51 homologue of Schizosaccharomyces pombe.

D F Muris 1, K Vreeken 1, A M Carr 1, B C Broughton 1, A R Lehmann 1, P H Lohman 1, A Pastink 1
PMCID: PMC311194  PMID: 8233794

Abstract

The RAD51 gene of Saccharomyces cerevisiae encodes a RecA like protein, which is involved in the recombinational repair of double strand breaks. We have isolated the RAD51 homologue, rhp51+, of the distantly related yeast strain Schizosaccharomyces pombe by heterologous hybridization. DNA sequence analysis of the rhp51+ gene revealed an open reading frame of 365 amino acids. Comparison of the amino acid sequences of RAD51 and rhp51+ showed a high level of conservation: 69% identical amino acids. There are two Mlul sites in the upstream region which may be associated with cell cycle regulation of the rhp51+ gene. The rhp51+ null allele, constructed by disruption of the coding region, is extremely sensitive to X-rays, indicating that the rhp51+ gene, like RAD51, is also involved in the repair of X-ray damage. The structural and functional homology between rhp51+ and RAD51 suggests evolutionary conservation of certain steps in the recombinational repair pathway.

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Selected References

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