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. 1993 Sep 25;21(19):4599–4603. doi: 10.1093/nar/21.19.4599

Reduced synonymous substitution rate at the start of enterobacterial genes.

A Eyre-Walker 1, M Bulmer 1
PMCID: PMC311196  PMID: 8233796

Abstract

Synonymous codon usage is less biased at the start of Escherichia coli genes than elsewhere. The rate of synonymous substitution between E.coli and Salmonella typhimurium is substantially reduced near the start of the gene, which suggests the presence of an additional selection pressure which competes with the selection for codons which are most rapidly translated. Possible competing sources of selection are the presence of secondary ribosome binding sites downstream from the start codon, the avoidance of mRNA secondary structure near the start of the gene and the use of sub-optimal codons to regulate gene expression. We provide evidence against the last of these possibilities. We also show that there is a decrease in the frequency of A, and an increase in the frequency of G along the E.coli genes at all three codon positions. We argue that these results are most consistent with selection to avoid mRNA secondary structure.

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Selected References

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