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. 1993 Oct 25;21(21):4941–4947. doi: 10.1093/nar/21.21.4941

Kappa immunoglobulin promoters and enhancers display developmentally controlled interactions.

R Fulton 1, B Van Ness 1
PMCID: PMC311410  PMID: 8177743

Abstract

We have investigated the interaction of the kappa immunoglobulin light chain intron and 3' enhancers with two different kappa promoters at distinct stages of B-cell development. We find that transiently transfected reporter gene constructs driven by either the kappa V-region promoter, or the kappa germline promoter, are controlled by the known enhancers of the locus in a developmentally regulated fashion. We have, however, observed differences in promoter activation by each enhancer. Moreover, constructs controlled by a combination of both enhancers are synergistically activated at the B-cell and plasma cell stages as compared with constructs containing either enhancer alone. This synergy is not observed early in development, at the pre-B cell stage. The pattern of enhancer and promoter interactions is discussed in the context of the known developmental regulation of the locus.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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