Expression of nestin and SOX2 in representative samples of NS1 and TS1 cells under different culture conditions for 7 days. NSCs cultured in NSC medium (A), GIC medium (B), or GIC medium + 5% FBS (C) and GICs cultured in NSC medium (D), GIC medium (E), or GIC medium + 5% FBS (F). Scale bar, 50 µm.