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Reversibility of serum-induced differentiation of TS1 cells cultured in GIC medium + 5% serum for 7 days and after that in GIC medium for 24 hours (A–D), or GIC medium for 7 days (E–H), or GIC medium + 5% FBS (reference cells) for 7 days (I–L). (A, E, I) Phase-contrast showing cell morphology and expression of HA (tag on viral transduced PDGF-B) and Brdu (B, F, J), SOX2 and nestin (C, G, K), and GFAP and Tuj1 (D, H, L). Scale bar, 50 µm.
