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. 2011 May 21;60(6):1716–1725. doi: 10.2337/db10-1229

TABLE 1.

The T-cell clone GAD35Za produces IL-13, IL-5, and IFN-γ

IL-13 ng/mL IL-5 ng/mL IFN-γ μg/mL IL-4 ng/mL IL-10 ng/mL
GAD35Za* 13.5 1.75 308 0 0
OVA-1* 0 0 130 0.5 0

*I-Ag7–restricted T clones GAD35Za and OVA-1 were plated (5 × 104/well) with irradiated syngeneic spleen cells (5 × 105/well) in the presence or absence of cognate antigen, 10 μg/mL of p524-538 or 50 μg/mL of ovalbumin, respectively. Forty-eight hours later, the supernatants were collected and cytokine levels determined by ELISA, as described in research design and methods, with background levels subtracted (T cells + antigen-presenting cells).