TABLE 1.
IL-13 ng/mL | IL-5 ng/mL | IFN-γ μg/mL | IL-4 ng/mL | IL-10 ng/mL | |
---|---|---|---|---|---|
GAD35Za* | 13.5 | 1.75 | 308 | 0 | 0 |
OVA-1* | 0 | 0 | 130 | 0.5 | 0 |
*I-Ag7–restricted T clones GAD35Za and OVA-1 were plated (5 × 104/well) with irradiated syngeneic spleen cells (5 × 105/well) in the presence or absence of cognate antigen, 10 μg/mL of p524-538 or 50 μg/mL of ovalbumin, respectively. Forty-eight hours later, the supernatants were collected and cytokine levels determined by ELISA, as described in research design and methods, with background levels subtracted (T cells + antigen-presenting cells).