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. 1986 Jun 11;14(11):4591–4603. doi: 10.1093/nar/14.11.4591

The human CK gene segment and the kappa deleting element are closely linked.

H G Klobeck, H G Zachau
PMCID: PMC311467  PMID: 3086844

Abstract

The element which mediates the deletion of the CK gene segment (abbreviations ref. 1) in human lambda light chain producing B-cells was found to be located 24 kb downstream of CK. The kappa deleting element is flanked by hepta- and nonanucleotide recognition sequences similar to the ones adjacent to the JK gene segments. Complementary recognition sequences with a 30 bp spacer were found in the JK-CK intron. For the first time the two partners of a recombination event in a mammalian immunoglobulin gene system have been linked on a physical map. The orientation of the recombination signals of the intron and the Kde sequences allows a joining by a simple deletion mechanism. Similarities and possible differences to the mechanism of V-J joining are discussed.

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Selected References

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