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. 1986 Jun 11;14(11):4605–4616. doi: 10.1093/nar/14.11.4605

Cloning a selected fragment from a human DNA 'fingerprint': isolation of an extremely polymorphic minisatellite.

Z Wong, V Wilson, A J Jeffreys, S L Thein
PMCID: PMC311468  PMID: 2423969

Abstract

A large hypervariable DNA fragment from a human DNA fingerprint was purified by preparative gel electrophoresis and molecular cloning. The cloned fragment contained a 6.3 kb long minisatellite consisting of multiple copies of a 37 bp repeat unit. Each repeat contained an 11 bp copy of the "core" sequences, a putative recombination signal in human DNA. The cloned minisatellite hybridized to a single locus in the human genome. This locus is extremely polymorphic, with at least 77 different alleles containing 14 to 525 repeat units per allele being resolved in a sample of 79 individuals. All alleles except the shortest are rare and the resulting heterozygosity is very high (approximately 97%). Cloned minisatellites should therefore provide a panel of extremely informative locus-specific probes ideal for linkage analysis in man.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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