Skip to main content
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1986 Jun 11;14(11):4683–4690. doi: 10.1093/nar/14.11.4683

A new method for predicting signal sequence cleavage sites.

G von Heijne
PMCID: PMC311474  PMID: 3714490

Abstract

A new method for identifying secretory signal sequences and for predicting the site of cleavage between a signal sequence and the mature exported protein is described. The predictive accuracy is estimated to be around 75-80% for both prokaryotic and eukaryotic proteins.

Full text

PDF
4683

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Flinta C., Persson B., Jörnvall H., von Heijne G. Sequence determinants of cytosolic N-terminal protein processing. Eur J Biochem. 1986 Jan 2;154(1):193–196. doi: 10.1111/j.1432-1033.1986.tb09378.x. [DOI] [PubMed] [Google Scholar]
  2. Klapper M. H. The independent distribution of amino acid near neighbor pairs into polypeptides. Biochem Biophys Res Commun. 1977 Oct 10;78(3):1018–1024. doi: 10.1016/0006-291x(77)90523-x. [DOI] [PubMed] [Google Scholar]
  3. Kuhn A., Wickner W. Conserved residues of the leader peptide are essential for cleavage by leader peptidase. J Biol Chem. 1985 Dec 15;260(29):15914–15918. [PubMed] [Google Scholar]
  4. McGeoch D. J. On the predictive recognition of signal peptide sequences. Virus Res. 1985 Oct;3(3):271–286. doi: 10.1016/0168-1702(85)90051-6. [DOI] [PubMed] [Google Scholar]
  5. Perlman D., Halvorson H. O. A putative signal peptidase recognition site and sequence in eukaryotic and prokaryotic signal peptides. J Mol Biol. 1983 Jun 25;167(2):391–409. doi: 10.1016/s0022-2836(83)80341-6. [DOI] [PubMed] [Google Scholar]
  6. Staden R. Computer methods to locate signals in nucleic acid sequences. Nucleic Acids Res. 1984 Jan 11;12(1 Pt 2):505–519. doi: 10.1093/nar/12.1part2.505. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. von Heijne G. How signal sequences maintain cleavage specificity. J Mol Biol. 1984 Feb 25;173(2):243–251. doi: 10.1016/0022-2836(84)90192-x. [DOI] [PubMed] [Google Scholar]
  8. von Heijne G. Patterns of amino acids near signal-sequence cleavage sites. Eur J Biochem. 1983 Jun 1;133(1):17–21. doi: 10.1111/j.1432-1033.1983.tb07424.x. [DOI] [PubMed] [Google Scholar]
  9. von Heijne G. Signal sequences. The limits of variation. J Mol Biol. 1985 Jul 5;184(1):99–105. doi: 10.1016/0022-2836(85)90046-4. [DOI] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES