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Plant Signaling & Behavior logoLink to Plant Signaling & Behavior
. 2010 Jul 1;5(7):896–898. doi: 10.4161/psb.5.7.12094

Localization of the arabidopsis histidine phosphotransfer proteins is independent of cytokinin

Jayson A Punwani 1, Joseph J Kieber 1,
PMCID: PMC3115040  PMID: 20484973

Abstract

Cytokinins are a class of mitogenic plant hormones that influence shoot and root growth, vascular and photomorphogenic development, leaf senescence, and many other aspects of plant growth and development. The Arabidopsis histidine phosphotransfer proteins (AHPs) play an important role in cytokinin signaling by bridging the perception of cytokinins by plasma-membrane receptors to the activation of cytokinin-responsive transcription factors. Based on previous microscopic observations, a model was developed in which the AHPs were thought to relocalize from the cytosol into the nucleus in response to exogenous cytokinin. However, analysis and quantification of the intracellular distribution of AHPs in both protoplasts and intact transgenic plants revealed that the subcellular localization of the AHPs is persistently nucleo-cytosolic and non-responsive to the state of the cytokinin response pathway. Here, we review and extend these findings and discuss their implications.

Key words: cytokinin, two-component signaling, nuclear-cytosolic movement, histidine phosphotransfer, plant hormones


Cytokinins are a group of plant hormones that play an important role in plant growth and development. The cytokinin signal transduction pathway consists of a two-component phosphorelay signal transduction pathway that conveys extracellular information to the nucleus.13 Signaling is initiated when cytokinin binds the extracellular domain of the Arabidopsis histidine kinase (AHKs) receptors. This results in autophosphorylation of the AHKs within their intracellular histidine-kinase domain. Phosphoryl groups are then transferred to cytosolic Arabidopsis histidine phosphotransfer proteins (AHPs), which were suggested to translocate to the nucleus in response to cytokinin treatment. The AHPs then transfer the phosphoryl groups to primarily nuclear-localized response regulators (type-A and type-B ARRs).

Two component response pathways are ubiquitous in prokaryotic cells.4,5 As this signaling module was co-opted by eukaryotic cells, it had to be adapted to the presence of the nuclear envelope and subsequent separation of nuclear and cytosolic signaling components. The AHPs play an important role in accommodating nucleocytosolic separation because they link the plasma membrane-localized receptors to the nuclear-localized response regulators. Previous models for cytokinin signaling suggested that AHP subcellular localization is tied to cytokinin signaling status: AHPs were thought to be localized to the cytosol in unstimulated cells and localized to the nucleus when cells were exposed to exogenous cytokinin.6,7 We investigated the localization of the AHP proteins in protoplasts and intact plants in which the cytokinin response pathway was activated or impaired. We found that bulk AHP subcellular localization is not altered by the status of cytokinin signaling. Instead, we propose that AHP proteins are constantly cycling between the nucleus and cytosol via an active transport mechanism.8

The Overall Distribution of AHPs is Actively Maintained as Nucleo-Cytosolic

To determine the localization of the AHPs in intact plants, we analyzed AHP2-GFP and AHP5-GFP protein fusions driven from native AHP promoters. In all tissues examined, AHPs were found in the nucleus and cytosol. Treatment with exogenous cytokinin did not affect this localization pattern, indicating that subcellular localization of AHPs in planta is independent of cytokinin signaling. In an attempt to repeat the initial observations of AHP movement in response to exogenous cytokinin, we analyzed the localization of AHP1, AHP2 and AHP5 in Arabidopsis mesophyll protoplasts. In wild-type protoplasts, these AHPs were typically found in the both the nucleus and cytosol and its distribution was not altered by treatment with exogenous cytokinins.8 Here, we extended this analysis to include AHP4, whose amino acid sequence is less similar to the other AHP proteins.9 Like all other AHPs tested, AHP4 was typically found in both the nucleus and cytosol, and this pattern was not altered by treatment with trans-zeatin (Fig. 1). We also examined the localization of AHPs in protoplasts obtained from mutant plants defective in upstream components of the cytokinin signaling pathway and again observed that the AHPs were found in both the nucleus and cytosol.8 Finally, to explore the mechanism of nuclear-cytosolic transport, we deleted portions of the AHP proteins and showed that domains within the AHP proteins are responsible for active nuclear import and export.8

Figure 1.

Figure 1

Analysis of the subcellular distribution of AHP4-GFP in wild-type Arabidopsis mesophyll protoplasts. (A–D) Representative images of GFP fluorescence signals from protoplasts transfected with plasmids encoding AHP4-GFP fusion proteins that have been treated with either water (control) or 1 µM trans-zeatin for 1 hr. (E) Quantification of data from (A–D). Values are the mean of three independent experiments, error bars indicate SE. Scale bars in (A and C) are 50 µm, scale bars in (B and D) are 25 µm. Methods are as described.8

Do AHPs Bring Phosphoryl Groups from the Nucleus to the Cytosol?

Our results suggest that AHP proteins are constantly cycling between the nucleus and cytosol, which may have interesting biochemical ramifications. The phosphoryl groups that participate in two-component signaling are linked by high-energy bonds, therefore the energy involved in phosphotransfer is close to zero.5,10 Furthermore, the histidine phosphotransfer domains (HPt) of the AHPs do not exhibit catalytic activity, but rather serve essentially as high energy phospho-donors for the receiver domains of the response regulators.4,5 A consequence of this is that phosphotransfer is potentially bidirectional in two-component phosphorelays. One example of such a reverse phosphorelay is the Escherichia coli Arc two-component system that senses anoxic or low redox conditions. In this system, the phosphorylated ArcA response regulator is inactivated via reverse flow of phosphate from ArcA through an Hpt domain to the Asp residue in the receiver domain of the ArcB tripartite sensor kinase receptor.11 In a similar manner, the AHPs could act as phosphatases, playing a role in attenuating cytokinin signaling by removing phosphoryl groups from ARRs when exogenous cytokinin levels are low and potentially shuttling the phosphoryl group back to the Asp residue on the receiver domain of the AHKs. The phosphorylation status of the AHKs, AHPs and ARRs might thus reach equilibrium that is proportional to the concentration of exogenous cytokinin (Fig. 2A).

Figure 2.

Figure 2

Potential implications of these observations. (A) AHPs may act as phosphatases and remove phosphoryl groups from ARRs. In this model, AHPs would serve to dampen the cytokinin signal if exogenous cytokinin levels were reduced. (B) Cytosolic-localized AHPs may donate phosphoryl groups to cytosolic-localized ARRs or other unkown cytoplasmic targets. (C) Unknown signals could modify AHPs to prevent or enhance AHP entry into the nucleus. As shown, AHPs movement is inhibited, perhaps resulting in reduced sensitivity to cytokinin. Filled arrowheads indicate cytokinin binding, open arrowheads indicate transfer of phosphoryl groups, dashed arrows indicate potential phosphotransfer to AHKs, bidirectional gray arrows indicate AHP movement into and out of the nucleus, and blocked arrowheads indicate inhibition of AHP movement.

Do Phosphorylated AHPs have a Function in the Cytosol?

Our observation that AHP-GFP fusion proteins are found in the cytosol even upon cytokinin stimulation suggests that phosphorylated AHPs may be present in the cytosol. Several groups have reported that at least some ARR proteins are in part localized to the cytosol.7,1214 Therefore, another intriguing consequence of our observations is that cytosolic AHPs may transfer phosphoryl groups to cytosolic-localized ARRs (Fig. 2B). This also raises the possibility that the AHPs could interact with additional cytoplasmic targets in a phosphor-dependent manner.

Does the AHP Active Transport Mechanism Represent a New Point of Control for Cytokinin Signaling?

Our results indicate that overall AHP subcellular localization is consistently nucleo-cytoplasmic and is not regulated by cytokinin. Interestingly, our data suggest the localization of the AHP proteins appears to be regulated by an unknown active transport mechanism. These observations have several implications for how the cytokinin-signaling pathway could be regulated during plant growth and development. Broadly speaking, this active transport mechanism may be manipulated to control the rate of AHP transport into or out of the nucleus, which in turn, would alter the sensitivity and response to cytokinin (Fig. 2C). For example, it is well established that auxin and cytokinin have an antagonistic relationship in many aspects of plant growth and development. An attractive hypothesis is that certain components of the auxin-signaling pathway directly interfere with the kinetics of AHP transport, resulting in an attenuated cytokinin signaling response. Another possibility is that the AHP active transport mechanism is developmentally and/or spatially regulated, resulting in distinct cytokinin sensitivity across different phases of growth or amongst different organs and tissues. Although the cytokinin pathway is arguably the most well characterized plant-hormone signal transduction cascade, the mechanisms modulating intracellular cytokinin response remain to be fully elucidated.

Acknowledgements

This work was supported by grants from the United States Department of Agriculture, The Department of Energy, and the National Science Foundation to J.J.K.

Addendum to: Punwani JA, Hutchison CE, Schaller GE, Kieber JK. The subcellular distribution of the Arabidopsis histidine phosphotransfer proteins is independent of cytokinin signaling. Plant J. 2010;62:473–482. doi: 10.1111/j.1365-313X.2010.04165.x.

Footnotes

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