. Author manuscript; available in PMC: 2012 Jun 21.

Published in final edited form as: Biochemistry. 2011 May 26;50(24):5426–5435. doi: 10.1021/bi200377c

Table 3.

Various solution concentrations chosen for H/DX to determine the order of binding of Ca²⁺ with troponin C. The values are calculated fractional species of each Ca²⁺-bound TnC species and are taken from the fractional species curves in Figure 3.

[Ca] μM	TnC-0Ca	TnC-1Ca	TnC-2Ca	TnC-3Ca	TnC-4Ca
0	100.0%	0.0%	0.0%	0.0%	0.0%
8.4	7.4%	19.7%	44.5%	23.7%	4.7%
10.4	2.3%	10.4%	39.8%	35.6%	11.9%
12.1	0.0%	4.9%	29.7%	42.6%	22.8%
13.8	0.0%	0.0%	18.2%	43.1%	38.7%
16.7	0.0%	0.0%	0.0%	29.1%	70.9%
2000	0.0%	0.0%	0.0%	0.0%	100.0%