Abstract
A small number of cellular proteins accumulate to high levels in cells infected with Herpes Simplex Virus (HSV) despite a generalised repression of most host cell bio-synthesis. An antibody to one such protein has been used to screen a lambda gt11 library and for polysome immunoprecipitation in order to isolate cDNA clones derived from the corresponding gene. The cDNA clones have been used in dot blot and nuclear run-off assays to show that HSV, like other DNA tumour viruses can transcriptionally induce a cellular gene. The mechanism of this effect which is dependent on viral protein synthesis and its possible significance in transformation by HSV are discussed.
Full text
PDF![5629](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae22/311581/7f514d20cbca/nar00283-0064.png)
![5630](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae22/311581/efe305ea8891/nar00283-0065.png)
![5631](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae22/311581/de090b08cae7/nar00283-0066.png)
![5632](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae22/311581/491e6c34174b/nar00283-0067.png)
![5633](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae22/311581/dbe2853f109d/nar00283-0068.png)
![5634](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae22/311581/ec6537264ddf/nar00283-0069.png)
![5635](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae22/311581/4f4c864ad186/nar00283-0070.png)
![5636](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae22/311581/912f8771147f/nar00283-0071.png)
![5637](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae22/311581/e24dc7b9c930/nar00283-0072.png)
![5638](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae22/311581/32e0e8f223c8/nar00283-0073.png)
![5639](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae22/311581/2c40aa1c9b35/nar00283-0074.png)
![5640](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae22/311581/0f5ba0f2beb9/nar00283-0075.png)
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Aviv H., Leder P. Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose. Proc Natl Acad Sci U S A. 1972 Jun;69(6):1408–1412. doi: 10.1073/pnas.69.6.1408. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Benton W. D., Davis R. W. Screening lambdagt recombinant clones by hybridization to single plaques in situ. Science. 1977 Apr 8;196(4286):180–182. doi: 10.1126/science.322279. [DOI] [PubMed] [Google Scholar]
- Cameron I. R., Park M., Dutia B. M., Orr A., Macnab J. C. Herpes simplex virus sequences involved in the initiation of oncogenic morphological transformation of rat cells are not required for maintenance of the transformed state. J Gen Virol. 1985 Mar;66(Pt 3):517–527. doi: 10.1099/0022-1317-66-3-517. [DOI] [PubMed] [Google Scholar]
- Everett R. D., Dunlop M. Trans activation of plasmid-borne promoters by adenovirus and several herpes group viruses. Nucleic Acids Res. 1984 Aug 10;12(15):5969–5978. doi: 10.1093/nar/12.15.5969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Galloway D. A., McDougall J. K. The oncogenic potential of herpes simplex viruses: evidence for a 'hit-and-run' mechanism. Nature. 1983 Mar 3;302(5903):21–24. doi: 10.1038/302021a0. [DOI] [PubMed] [Google Scholar]
- Green M. R., Treisman R., Maniatis T. Transcriptional activation of cloned human beta-globin genes by viral immediate-early gene products. Cell. 1983 Nov;35(1):137–148. doi: 10.1016/0092-8674(83)90216-7. [DOI] [PubMed] [Google Scholar]
- Greenberg M. E., Ziff E. B. Stimulation of 3T3 cells induces transcription of the c-fos proto-oncogene. Nature. 1984 Oct 4;311(5985):433–438. doi: 10.1038/311433a0. [DOI] [PubMed] [Google Scholar]
- Imperiale M. J., Feldman L. T., Nevins J. R. Activation of gene expression by adenovirus and herpesvirus regulatory genes acting in trans and by a cis-acting adenovirus enhancer element. Cell. 1983 Nov;35(1):127–136. doi: 10.1016/0092-8674(83)90215-5. [DOI] [PubMed] [Google Scholar]
- Inglis S. C. Inhibition of host protein synthesis and degradation of cellular mRNAs during infection by influenza and herpes simplex virus. Mol Cell Biol. 1982 Dec;2(12):1644–1648. doi: 10.1128/mcb.2.12.1644. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jones N., Shenk T. An adenovirus type 5 early gene function regulates expression of other early viral genes. Proc Natl Acad Sci U S A. 1979 Aug;76(8):3665–3669. doi: 10.1073/pnas.76.8.3665. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kao H. T., Nevins J. R. Transcriptional activation and subsequent control of the human heat shock gene during adenovirus infection. Mol Cell Biol. 1983 Nov;3(11):2058–2065. doi: 10.1128/mcb.3.11.2058. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Keller J. M., Alwine J. C. Activation of the SV40 late promoter: direct effects of T antigen in the absence of viral DNA replication. Cell. 1984 Feb;36(2):381–389. doi: 10.1016/0092-8674(84)90231-9. [DOI] [PubMed] [Google Scholar]
- LaThangue N. B., Shriver K., Dawson C., Chan W. L. Herpes simplex virus infection causes the accumulation of a heat-shock protein. EMBO J. 1984 Feb;3(2):267–277. doi: 10.1002/j.1460-2075.1984.tb01796.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MACPHERSON I., STOKER M. Polyoma transformation of hamster cell clones--an investigation of genetic factors affecting cell competence. Virology. 1962 Feb;16:147–151. doi: 10.1016/0042-6822(62)90290-8. [DOI] [PubMed] [Google Scholar]
- Macnab J. C., Orr A., La Thangue N. B. Cellular proteins expressed in herpes simplex virus transformed cells also accumulate on herpes simplex virus infection. EMBO J. 1985 Dec 1;4(12):3223–3228. doi: 10.1002/j.1460-2075.1985.tb04069.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Murphy D., Brickell P. M., Latchman D. S., Willison K., Rigby P. W. Transcripts regulated during normal embryonic development and oncogenic transformation share a repetitive element. Cell. 1983 Dec;35(3 Pt 2):865–871. doi: 10.1016/0092-8674(83)90119-8. [DOI] [PubMed] [Google Scholar]
- O'Hare P., Hayward G. S. Three trans-acting regulatory proteins of herpes simplex virus modulate immediate-early gene expression in a pathway involving positive and negative feedback regulation. J Virol. 1985 Dec;56(3):723–733. doi: 10.1128/jvi.56.3.723-733.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Petersen N. S., Mitchell H. K. Recovery of protein synthesis after heat shock: prior heat treatment affects the ability of cells to translate mRNA. Proc Natl Acad Sci U S A. 1981 Mar;78(3):1708–1711. doi: 10.1073/pnas.78.3.1708. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schneider C., Kurkinen M., Greaves M. Isolation of cDNA clones for the human transferrin receptor. EMBO J. 1983;2(12):2259–2263. doi: 10.1002/j.1460-2075.1983.tb01732.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Soprano K. J., Jonak G. J., Galanti N., Floros J., Baserga R. Identification of an SV40 DNA sequence related to the reactivation of silent rRNA genes in human greater than mouse hybrid cells. Virology. 1981 Feb;109(1):127–136. doi: 10.1016/0042-6822(81)90477-3. [DOI] [PubMed] [Google Scholar]
- Stenberg R. M., Pizer L. I. Herpes simplex virus-induced changes in cellular and adenovirus RNA metabolism in an adenovirus type 5-transformed human cell line. J Virol. 1982 May;42(2):474–487. doi: 10.1128/jvi.42.2.474-487.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wu C. The 5' ends of Drosophila heat shock genes in chromatin are hypersensitive to DNase I. Nature. 1980 Aug 28;286(5776):854–860. doi: 10.1038/286854a0. [DOI] [PubMed] [Google Scholar]