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. 1986 Aug 11;14(15):6017–6030. doi: 10.1093/nar/14.15.6017

Restriction endonuclease activity induced by NC-1A virus infection of a Chlorella-like green alga.

Y Xia, D E Burbank, J L Van Etten
PMCID: PMC311618  PMID: 3018667

Abstract

A type II restriction endonuclease, CviBI, was isolated from a eukaryotic, Chlorella-like green alga infected with the dsDNA containing virus NC-1A. The enzyme recognizes the sequence GANTC and cleaves DNA between the G and A. Methylation of deoxyadenosine in the GANTC sequence probably inhibits enzyme activity. In vitro CviBI cleaves host nuclear DNA but not viral DNA. A survey of 18 other viruses which infect the same Chlorella sp. revealed that infection with 5 of these viruses also induced a restriction endonuclease which cleaves DNA into the same size fragments as CviBI.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Boyer H., Scibienski E., Slocum H., Roulland-Dussoix D. The in vitro restriction of the replicative form of W.T. and mutant fd phage DNA. Virology. 1971 Dec;46(3):703–710. doi: 10.1016/0042-6822(71)90072-9. [DOI] [PubMed] [Google Scholar]
  2. Kauc L., Piekarowicz A. Purification and properties of a new restriction endonuclease from Haemophilus influenzae Rf. Eur J Biochem. 1978 Dec;92(2):417–426. doi: 10.1111/j.1432-1033.1978.tb12762.x. [DOI] [PubMed] [Google Scholar]
  3. Marinus M. G., Carraway M., Frey A. Z., Brown L., Arraj J. A. Insertion mutations in the dam gene of Escherichia coli K-12. Mol Gen Genet. 1983;192(1-2):288–289. doi: 10.1007/BF00327681. [DOI] [PubMed] [Google Scholar]
  4. McClelland M., Nelson M. The effect of site specific methylation on restriction endonuclease digestion. Nucleic Acids Res. 1985;13 (Suppl):r201–r207. doi: 10.1093/nar/13.suppl.r201. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Schleif R. Assaying of organisms for the presence of restriction endonucleases. Methods Enzymol. 1980;65(1):19–23. doi: 10.1016/s0076-6879(80)65004-6. [DOI] [PubMed] [Google Scholar]
  6. Schuster A. M., Burbank D. E., Meister B., Skrdla M. P., Meints R. H., Hattman S., Swinton D., Van Etten J. L. Characterization of viruses infecting a eukaryotic Chlorella-like green alga. Virology. 1986 Apr 15;150(1):170–177. doi: 10.1016/0042-6822(86)90276-x. [DOI] [PubMed] [Google Scholar]
  7. Van Etten J. L., Burbank D. E., Schuster A. M., Meints R. H. Lytic viruses infecting a Chlorella-like alga. Virology. 1985 Jan 15;140(1):135–143. doi: 10.1016/0042-6822(85)90452-0. [DOI] [PubMed] [Google Scholar]
  8. Van Etten J. L., Schuster A. M., Girton L., Burbank D. E., Swinton D., Hattman S. DNA methylation of viruses infecting a eukaryotic Chlorella-like green alga. Nucleic Acids Res. 1985 May 24;13(10):3471–3478. doi: 10.1093/nar/13.10.3471. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Xia Y. N., Burbank D. E., Uher L., Rabussay D., Van Etten J. L. Restriction endonuclease activity induced by PBCV-1 virus infection of a Chlorella-like green alga. Mol Cell Biol. 1986 May;6(5):1430–1439. doi: 10.1128/mcb.6.5.1430. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Xia Y. N., Van Etten J. L. DNA methyltransferase induced by PBCV-1 virus infection of a Chlorella-like green alga. Mol Cell Biol. 1986 May;6(5):1440–1445. doi: 10.1128/mcb.6.5.1440. [DOI] [PMC free article] [PubMed] [Google Scholar]

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