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. 1986 Aug 26;14(16):6375–6392. doi: 10.1093/nar/14.16.6375

Intron sequences modulate feather keratin gene transcription in Xenopus oocytes.

A M Koltunow, K Gregg, G E Rogers
PMCID: PMC311652  PMID: 2428013

Abstract

We have shown that chicken feather keratin genes are transcribed at extremely low levels in Xenopus oocytes. Primer extension reactions using carefully selected primers have shown that none of the detectable transcripts are spliced. When the single intron, located in the 5' untranslated region, was removed from the gene, we observed a 5-fold increase in transcript initiation from the in vivo "cap" site. Significantly, this 5-fold increase was also observed when the feather keratin intron was replaced with a similar sized fragment from pBR322. We conclude that the efficiency of accurate transcriptional initiation of chicken feather keratin genes in the oocyte is affected by DNA sequences within the intron.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aviv H., Leder P. Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose. Proc Natl Acad Sci U S A. 1972 Jun;69(6):1408–1412. doi: 10.1073/pnas.69.6.1408. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Brand A. H., Breeden L., Abraham J., Sternglanz R., Nasmyth K. Characterization of a "silencer" in yeast: a DNA sequence with properties opposite to those of a transcriptional enhancer. Cell. 1985 May;41(1):41–48. doi: 10.1016/0092-8674(85)90059-5. [DOI] [PubMed] [Google Scholar]
  3. Capecchi M. R. High efficiency transformation by direct microinjection of DNA into cultured mammalian cells. Cell. 1980 Nov;22(2 Pt 2):479–488. doi: 10.1016/0092-8674(80)90358-x. [DOI] [PubMed] [Google Scholar]
  4. De Robertis E. M., Mertz J. E. Coupled transcription-translation of DNA injected into Xenopus oocytes. Cell. 1977 Sep;12(1):175–182. doi: 10.1016/0092-8674(77)90195-7. [DOI] [PubMed] [Google Scholar]
  5. Green M. R., Maniatis T., Melton D. A. Human beta-globin pre-mRNA synthesized in vitro is accurately spliced in Xenopus oocyte nuclei. Cell. 1983 Mar;32(3):681–694. doi: 10.1016/0092-8674(83)90054-5. [DOI] [PubMed] [Google Scholar]
  6. Gurdon J. B. Methods for nuclear transplantation in amphibia. Methods Cell Biol. 1977;16:125–139. doi: 10.1016/s0091-679x(08)60096-5. [DOI] [PubMed] [Google Scholar]
  7. Kozak M. Comparison of initiation of protein synthesis in procaryotes, eucaryotes, and organelles. Microbiol Rev. 1983 Mar;47(1):1–45. doi: 10.1128/mr.47.1.1-45.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Laskey R. A., Mills A. D. Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography. Eur J Biochem. 1975 Aug 15;56(2):335–341. doi: 10.1111/j.1432-1033.1975.tb02238.x. [DOI] [PubMed] [Google Scholar]
  9. McKnight S. L., Gavis E. R., Kingsbury R., Axel R. Analysis of transcriptional regulatory signals of the HSV thymidine kinase gene: identification of an upstream control region. Cell. 1981 Aug;25(2):385–398. doi: 10.1016/0092-8674(81)90057-x. [DOI] [PubMed] [Google Scholar]
  10. McKnight S. L., Kingsbury R. C., Spence A., Smith M. The distal transcription signals of the herpesvirus tk gene share a common hexanucleotide control sequence. Cell. 1984 May;37(1):253–262. doi: 10.1016/0092-8674(84)90321-0. [DOI] [PubMed] [Google Scholar]
  11. McKnight S. L. The nucleotide sequence and transcript map of the herpes simplex virus thymidine kinase gene. Nucleic Acids Res. 1980 Dec 20;8(24):5949–5964. doi: 10.1093/nar/8.24.5949. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Molloy P. L., Powell B. C., Gregg K., Barone E. D., Rogers G. E. Organisation of feather keratin genes in the chick genome. Nucleic Acids Res. 1982 Oct 11;10(19):6007–6021. doi: 10.1093/nar/10.19.6007. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Powell B. C., Kemp D. J., Partington G. A., Gibbs P. E., Rogers G. E. Control of feather keratin synthesis by the availability of keratin mRNA. Biochem Biophys Res Commun. 1976 Feb 23;68(4):1263–1271. doi: 10.1016/0006-291x(76)90333-8. [DOI] [PubMed] [Google Scholar]
  14. Probst E., Kressmann A., Birnstiel M. L. Expression of sea urchin histone genes in the oocyte of Xenopus laevis. J Mol Biol. 1979 Dec 15;135(3):709–732. doi: 10.1016/0022-2836(79)90173-6. [DOI] [PubMed] [Google Scholar]
  15. Proudfoot N. J., Rutherford T. R., Partington G. A. Transcriptional analysis of human zeta globin genes. EMBO J. 1984 Jul;3(7):1533–1540. doi: 10.1002/j.1460-2075.1984.tb02007.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Rungger D., Türler H. DNAs of simian virus 40 and polyoma direct the synthesis of viral tumor antigens and capsid proteins in Xenopus oocytes. Proc Natl Acad Sci U S A. 1978 Dec;75(12):6073–6077. doi: 10.1073/pnas.75.12.6073. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Rusconi S., Schaffner W. Transformation of frog embryos with a rabbit beta-globin gene. Proc Natl Acad Sci U S A. 1981 Aug;78(8):5051–5055. doi: 10.1073/pnas.78.8.5051. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Sassone-Corsi P., Wildeman A., Chambon P. A trans-acting factor is responsible for the simian virus 40 enhancer activity in vitro. Nature. 1985 Feb 7;313(6002):458–463. doi: 10.1038/313458a0. [DOI] [PubMed] [Google Scholar]
  20. Schöler H. R., Gruss P. Specific interaction between enhancer-containing molecules and cellular components. Cell. 1984 Feb;36(2):403–411. doi: 10.1016/0092-8674(84)90233-2. [DOI] [PubMed] [Google Scholar]
  21. Wickens M. P., Gurdon J. B. Post-transcriptional processing of simian virus 40 late transcripts in injected frog oocytes. J Mol Biol. 1983 Jan 5;163(1):1–26. doi: 10.1016/0022-2836(83)90027-x. [DOI] [PubMed] [Google Scholar]
  22. Wickens M. P., Woo S., O'Malley B. W., Gurdon J. B. Expression of a chicken chromosomal ovalbumin gene injected into frog oocyte nuclei. Nature. 1980 Jun 26;285(5767):628–634. doi: 10.1038/285628a0. [DOI] [PubMed] [Google Scholar]
  23. Wigley P. L., Sturm R. A., Wells J. R. The tissue-specific chicken histone H5 gene is transcribed with fidelity in Xenopus laevis oocytes. J Mol Biol. 1985 Feb 5;181(3):449–452. doi: 10.1016/0022-2836(85)90231-1. [DOI] [PubMed] [Google Scholar]
  24. de Villiers J., Olson L., Banerji J., Schaffner W. Analysis of the transcriptional enhancer effect. Cold Spring Harb Symp Quant Biol. 1983;47(Pt 2):911–919. doi: 10.1101/sqb.1983.047.01.105. [DOI] [PubMed] [Google Scholar]

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