Skip to main content
PMC home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1986 Sep 11;14(17):7031–7045. doi: 10.1093/nar/14.17.7031

Restriction endonucleases from Herpetosiphon giganteus: an example of the evolution of DNA recognition specificity?

P R Whitehead, D Jacobs, N L Brown
PMCID: PMC311715  PMID: 3020503

Abstract

We describe the partial purification and characterisation of five Type II restriction endonucleases from two strains of Herpetosiphon giganteus. One of the activities, HgiJII, was the first enzyme found that cleaves DNA at the family of related sequences 5'-G-R-G-C-Y/C-3'. This enzyme may be related to the enzyme HgiAI from a different strain of the same species, and which cleaves at the sites 5'-G-W-G-C-W/C-3'. We have shown that DNAs from the strains producing HgiAI and HgiJII are resistant to both of these restriction endonucleases. The remaining four enzymes described here share recognition and cleavage specificities with other restriction endonucleases. The evolution of Type II restriction-modification systems and their role in vivo are discussed.

Full text

PDF
7031

Images in this article

7036Image
on p.7036
7037Image
on p.7037
7039Image
on p.7039

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brown N. L., McClelland M., Whitehead P. R. HgiAI: a restriction endonuclease from Herpetosiphon giganteus HP1023. Gene. 1980 Apr;9(1-2):49–68. doi: 10.1016/0378-1119(80)90166-3. [DOI] [PubMed] [Google Scholar]
  2. Brown N. L., Smith M. A general method for defining restriction enzyme cleavage and recognition sites. Methods Enzymol. 1980;65(1):391–404. doi: 10.1016/s0076-6879(80)65050-2. [DOI] [PubMed] [Google Scholar]
  3. Fuchs C., Rosenvold E. C., Honigman A., Szybalski W. Identification of palindromic sequences recognized by restriction endonucleases, as based on the tabularized sequencing data for seven viral and plasmid DNAs. Gene. 1980 Sep;10(4):357–370. doi: 10.1016/0378-1119(80)90156-0. [DOI] [PubMed] [Google Scholar]
  4. Kröger M., Hobom G., Schütte H., Mayer H. Eight new restriction endonucleases fröm Herpetosiphon giganteus--divergent evolution in a family of enzymes. Nucleic Acids Res. 1984 Apr 11;12(7):3127–3141. doi: 10.1093/nar/12.7.3127. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Krüger D. H., Bickle T. A. Bacteriophage survival: multiple mechanisms for avoiding the deoxyribonucleic acid restriction systems of their hosts. Microbiol Rev. 1983 Sep;47(3):345–360. doi: 10.1128/mr.47.3.345-360.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Maxam A. M., Gilbert W. Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980;65(1):499–560. doi: 10.1016/s0076-6879(80)65059-9. [DOI] [PubMed] [Google Scholar]
  7. May M. S., Hattman S. Analysis of bacteriophage deoxyribonucleic acid sequences methylated by host- and R-factor-controlled enzymes. J Bacteriol. 1975 Aug;123(2):768–770. doi: 10.1128/jb.123.2.768-770.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. McClelland M., Nelson M. The effect of site specific methylation on restriction endonuclease digestion. Nucleic Acids Res. 1985;13 (Suppl):r201–r207. doi: 10.1093/nar/13.suppl.r201. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Messing J., Crea R., Seeburg P. H. A system for shotgun DNA sequencing. Nucleic Acids Res. 1981 Jan 24;9(2):309–321. doi: 10.1093/nar/9.2.309. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Misra T. K., Brown N. L., Fritzinger D. C., Pridmore R. D., Barnes W. M., Haberstroh L., Silver S. Mercuric ion-resistance operons of plasmid R100 and transposon Tn501: the beginning of the operon including the regulatory region and the first two structural genes. Proc Natl Acad Sci U S A. 1984 Oct;81(19):5975–5979. doi: 10.1073/pnas.81.19.5975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Murray N. E., Murray K. Manipulation of restriction targets in phage lambda to form receptor chromosomes for DNA fragments. Nature. 1974 Oct 11;251(5475):476–481. doi: 10.1038/251476a0. [DOI] [PubMed] [Google Scholar]
  12. Oka A., Nomura N., Morita M., Sugisaki H., Sugimoto K., Takanami M. Nucleotide sequence of small ColE1 derivatives: structure of the regions essential for autonomous replication and colicin E1 immunity. Mol Gen Genet. 1979 May 4;172(2):151–159. doi: 10.1007/BF00268276. [DOI] [PubMed] [Google Scholar]
  13. Roberts R. J. Restriction and modification enzymes and their recognition sequences. Nucleic Acids Res. 1985;13 (Suppl):r165–r200. doi: 10.1093/nar/13.suppl.r165. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Rosenberg J. M., Boyer H. W., Greene P. The structure and function of the Eco RI restriction endonuclease. Gene Amplif Anal. 1981;1:131–164. [PubMed] [Google Scholar]
  15. Sanger F., Coulson A. R., Friedmann T., Air G. M., Barrell B. G., Brown N. L., Fiddes J. C., Hutchison C. A., 3rd, Slocombe P. M., Smith M. The nucleotide sequence of bacteriophage phiX174. J Mol Biol. 1978 Oct 25;125(2):225–246. doi: 10.1016/0022-2836(78)90346-7. [DOI] [PubMed] [Google Scholar]
  16. Sutcliffe J. G. Complete nucleotide sequence of the Escherichia coli plasmid pBR322. Cold Spring Harb Symp Quant Biol. 1979;43(Pt 1):77–90. doi: 10.1101/sqb.1979.043.01.013. [DOI] [PubMed] [Google Scholar]
  17. Whitehead P. R., Brown N. L. AhaIII: a restriction endonuclease with a recognition sequence containing only A:T basepairs. FEBS Lett. 1982 Jul 5;143(2):296–300. doi: 10.1016/0014-5793(82)80120-8. [DOI] [PubMed] [Google Scholar]
  18. Whitehead P. R., Brown N. L. EaeI: a restriction endonuclease from Enterobacter aerogenes. FEBS Lett. 1983 May 2;155(1):97–101. doi: 10.1016/0014-5793(83)80217-8. [DOI] [PubMed] [Google Scholar]
  19. Whitehead P. R., Brown N. L. Three restriction endonucleases from Anabaena flos-aquae. J Gen Microbiol. 1985 Apr;131(4):951–958. doi: 10.1099/00221287-131-4-951. [DOI] [PubMed] [Google Scholar]
  20. Wolfes H., Fliess A., Pingoud A. A comparison of the structural requirements for DNA cleavage by the isoschizomers HaeIII, BspRI and BsuRI. Eur J Biochem. 1985 Jul 1;150(1):105–110. doi: 10.1111/j.1432-1033.1985.tb08994.x. [DOI] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES