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. 1986 Sep 11;14(17):7115–7123. doi: 10.1093/nar/14.17.7115

Nucleotide sequence of the gene responsible for D-xylose uptake in Escherichia coli.

N Kurose, K Watanabe, A Kimura
PMCID: PMC311722  PMID: 3532033

Abstract

The nucleotide sequence of the cloned DNA, 363 bp in length, has been determined. It can complement the mutation of Escherichia coli having a decreased activity of D-xylose uptake at low temperature. Nucleotide sequence analysis found one possible reading frame coding for a polypeptide consisting of 61 amino acids. Several signal sequences conserved in the promoter regions of E. coli were found in the upstream regions of the open frame. This included the Shine-Dalgarno sequence, the Pribnow box, and the sequence conserved in the "-35 region" with a preferable spacing from each other for an efficient transcription. Downstream from the termination codon, the inverted repeat sequence was present, followed by 3 successive T's.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ahlem C., Huisman W., Neslund G., Dahms A. S. Purification and properties of a periplasmic D-xylose-binding protein from Escherichia coli K-12. J Biol Chem. 1982 Mar 25;257(6):2926–2931. [PubMed] [Google Scholar]
  2. David J. D., Wiesmeyer H. Control of xylose metabolism in Escherichia coli. Biochim Biophys Acta. 1970 Mar 24;201(3):497–499. doi: 10.1016/0304-4165(70)90171-6. [DOI] [PubMed] [Google Scholar]
  3. Davis E. O., Jones-Mortimer M. C., Henderson P. J. Location of a structural gene for xylose-H+ symport at 91 min on the linkage map of Escherichia coli K12. J Biol Chem. 1984 Feb 10;259(3):1520–1525. [PubMed] [Google Scholar]
  4. Hawley D. K., McClure W. R. Compilation and analysis of Escherichia coli promoter DNA sequences. Nucleic Acids Res. 1983 Apr 25;11(8):2237–2255. doi: 10.1093/nar/11.8.2237. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Lam V. M., Daruwalla K. R., Henderson P. J., Jones-Mortimer M. C. Proton-linked D-xylose transport in Escherichia coli. J Bacteriol. 1980 Jul;143(1):396–402. doi: 10.1128/jb.143.1.396-402.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Lawlis V. B., Dennis M. S., Chen E. Y., Smith D. H., Henner D. J. Cloning and sequencing of the xylose isomerase and xylulose kinase genes of Escherichia coli. Appl Environ Microbiol. 1984 Jan;47(1):15–21. doi: 10.1128/aem.47.1.15-21.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Rosenberg M., Court D. Regulatory sequences involved in the promotion and termination of RNA transcription. Annu Rev Genet. 1979;13:319–353. doi: 10.1146/annurev.ge.13.120179.001535. [DOI] [PubMed] [Google Scholar]
  8. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Schellenberg G. D., Sarthy A., Larson A. E., Backer M. P., Crabb J. W., Lidstrom M., Hall B. D., Furlong C. E. Xylose isomerase from Escherichia coli. Characterization of the protein and the structural gene. J Biol Chem. 1984 Jun 10;259(11):6826–6832. [PubMed] [Google Scholar]
  10. Shamanna D. K., Sanderson K. E. Genetics and regulation of D-xylose utilization in Salmonella typhimurium LT2. J Bacteriol. 1979 Jul;139(1):71–79. doi: 10.1128/jb.139.1.71-79.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Shamanna D. K., Sanderson K. E. Uptake and catabolism of D-xylose in Salmonella typhimurium LT2. J Bacteriol. 1979 Jul;139(1):64–70. doi: 10.1128/jb.139.1.64-70.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Siebenlist U., Simpson R. B., Gilbert W. E. coli RNA polymerase interacts homologously with two different promoters. Cell. 1980 Jun;20(2):269–281. doi: 10.1016/0092-8674(80)90613-3. [DOI] [PubMed] [Google Scholar]
  13. Stevens F. J., Wu T. T. Growth on D-lyxose of a mutant strain of Escherichia coli K12 using a novel isomerase and enzymes related to D-xylase metabolism. J Gen Microbiol. 1976 Dec;97(2):257–265. doi: 10.1099/00221287-97-2-257. [DOI] [PubMed] [Google Scholar]
  14. Tso J. Y., Zalkin H., van Cleemput M., Yanofsky C., Smith J. M. Nucleotide sequence of Escherichia coli purF and deduced amino acid sequence of glutamine phosphoribosylpyrophosphate amidotransferase. J Biol Chem. 1982 Apr 10;257(7):3525–3531. [PubMed] [Google Scholar]
  15. Wu T. T. Growth of a mutant of Escherichia coli K-12 on xylitol by recruiting enzymes for D-xylose and L1,2-propanediol metabolism. Biochim Biophys Acta. 1976 May 28;428(3):656–663. doi: 10.1016/0304-4165(76)90195-1. [DOI] [PubMed] [Google Scholar]

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