Table 1.

Summary of the Equations Generated from the Standard Curves Between Sperm Concentration and Absorbance Measured at 400 nm by Use of a Microspectrophotometer, and the Associated Coefficient Factors (R²-Values) and Effective Absorbance Range for Sperm Samples Collected by Crushing of Dissected Testis or by Stripping of Live Males of Zebrafish Danio rerio, Green Swordtail Xiphophorus helleri, and Medaka Oryzias latipes^a

	Zebrafish		Green swordtail		Medaka
Values	Crushing of testis	Stripping of male	Crushing of testis	Stripping of male	Crushing of testis
Equation	Y = (3 × 108)X − 1 × 107	Y = (4 × 108)X − 3 × 107	Y = (3 × 108)X − 2 × 107	Y = (2 × 108)X − 4 × 106	Y = (3 × 108)X − 2 × 107
R2-value	0.9405	0.9170	0.9419	0.9800	0.9319
Absorbance range	0.034–0.936	0.028–0.961	0.014–1.154	0.038–1.082	0.041–0.896

^aStripping experiments were not performed for medaka.