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. 1986 Oct 10;14(19):7661–7674. doi: 10.1093/nar/14.19.7661

Hybridization triggered cross-linking of deoxyoligonucleotides.

T R Webb, M D Matteucci
PMCID: PMC311787  PMID: 3774542

Abstract

This paper reports details of the synthesis of oligodeoxynucleotides containing the modified base 5-methyl-N4,N4-ethanocytosine (Ce). The 9-fluorenylmethoxycarbonyl group is used as a protecting group for the exocyclic amines of dA and dC. This group can be removed rapidly under very mild conditions. Oligomers containing the Ce base form a cross-link when hybridized to their complementary deoxyoligonucleotides. Some of the scope and limitations of these cross-link forming oligonucleotides are reported.

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Selected References

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