Abstract
The nucleotide sequence of a 3120 bp region of the E. coli chromosome that includes the entire ptr gene has been determined. The proposed coding region for Protease III is 2889 nucleotides long, which would encode a protein consisting of 962 amino acids with a calculated molecular mass of 107,719 daltons. The predicted primary structure of the protein includes a 23-residue signal sequence, cleavage of which would give rise to a mature protein of molecular mass 105,124 daltons. At its 3' end, the ptr gene overlaps the start of the recB coding sequence by 8 bases, suggesting that these genes may form part of an operon.
Full text
PDF








Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Biggin M. D., Gibson T. J., Hong G. F. Buffer gradient gels and 35S label as an aid to rapid DNA sequence determination. Proc Natl Acad Sci U S A. 1983 Jul;80(13):3963–3965. doi: 10.1073/pnas.80.13.3963. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cheng Y. S., Zipser D., Cheng C. Y., Rolseth S. J. Isolation and characterization of mutations in the structural gene for protease III (ptr). J Bacteriol. 1979 Oct;140(1):125–130. doi: 10.1128/jb.140.1.125-130.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cheng Y. S., Zipser D. Purification and characterization of protease III from Escherichia coli. J Biol Chem. 1979 Jun 10;254(11):4698–4706. [PubMed] [Google Scholar]
- Dykstra C. C., Kushner S. R. Physical characterization of the cloned protease III gene from Escherichia coli K-12. J Bacteriol. 1985 Sep;163(3):1055–1059. doi: 10.1128/jb.163.3.1055-1059.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dykstra C. C., Prasher D., Kushner S. R. Physical and biochemical analysis of the cloned recB and recC genes of Escherichia coli K-12. J Bacteriol. 1984 Jan;157(1):21–27. doi: 10.1128/jb.157.1.21-27.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Finch P. W., Wilson R. E., Brown K., Hickson I. D., Tomkinson A. E., Emmerson P. T. Complete nucleotide sequence of the Escherichia coli recC gene and of the thyA-recC intergenic region. Nucleic Acids Res. 1986 Jun 11;14(11):4437–4451. doi: 10.1093/nar/14.11.4437. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gayda R. C., Stephens P. E., Hewick R., Schoemaker J. M., Dreyer W. J., Markovitz A. Regulatory region of the heat shock-inducible capR (lon) gene: DNA and protein sequences. J Bacteriol. 1985 Apr;162(1):271–275. doi: 10.1128/jb.162.1.271-275.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Goff S. A., Casson L. P., Goldberg A. L. Heat shock regulatory gene htpR influences rates of protein degradation and expression of the lon gene in Escherichia coli. Proc Natl Acad Sci U S A. 1984 Nov;81(21):6647–6651. doi: 10.1073/pnas.81.21.6647. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Goldberg A. L., Swamy K. H., Chung C. H., Larimore F. S. Proteases in Escherichia coli. Methods Enzymol. 1981;80(Pt 100):680–702. doi: 10.1016/s0076-6879(81)80052-3. [DOI] [PubMed] [Google Scholar]
- Grosjean H., Fiers W. Preferential codon usage in prokaryotic genes: the optimal codon-anticodon interaction energy and the selective codon usage in efficiently expressed genes. Gene. 1982 Jun;18(3):199–209. doi: 10.1016/0378-1119(82)90157-3. [DOI] [PubMed] [Google Scholar]
- Hawley D. K., McClure W. R. Compilation and analysis of Escherichia coli promoter DNA sequences. Nucleic Acids Res. 1983 Apr 25;11(8):2237–2255. doi: 10.1093/nar/11.8.2237. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hickson I. D., Atkinson K. E., Hutton L., Tomkinson A. E., Emmerson P. T. Molecular amplification and purification of the E. coli recC gene product. Nucleic Acids Res. 1984 May 11;12(9):3807–3819. doi: 10.1093/nar/12.9.3807. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Konigsberg W., Godson G. N. Evidence for use of rare codons in the dnaG gene and other regulatory genes of Escherichia coli. Proc Natl Acad Sci U S A. 1983 Feb;80(3):687–691. doi: 10.1073/pnas.80.3.687. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mulligan M. E., Hawley D. K., Entriken R., McClure W. R. Escherichia coli promoter sequences predict in vitro RNA polymerase selectivity. Nucleic Acids Res. 1984 Jan 11;12(1 Pt 2):789–800. doi: 10.1093/nar/12.1part2.789. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Phillips T. A., VanBogelen R. A., Neidhardt F. C. lon gene product of Escherichia coli is a heat-shock protein. J Bacteriol. 1984 Jul;159(1):283–287. doi: 10.1128/jb.159.1.283-287.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Queen C., Korn L. J. A comprehensive sequence analysis program for the IBM personal computer. Nucleic Acids Res. 1984 Jan 11;12(1 Pt 2):581–599. doi: 10.1093/nar/12.1part2.581. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Staden R. Measurements of the effects that coding for a protein has on a DNA sequence and their use for finding genes. Nucleic Acids Res. 1984 Jan 11;12(1 Pt 2):551–567. doi: 10.1093/nar/12.1part2.551. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Swamy K. H., Goldberg A. L. Subcellular distribution of various proteases in Escherichia coli. J Bacteriol. 1982 Mar;149(3):1027–1033. doi: 10.1128/jb.149.3.1027-1033.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Umeno M., Sasaki M., Anai M., Takagi Y. Properties of the recB and recC gene products of Escherichia coli. Biochem Biophys Res Commun. 1983 Nov 15;116(3):1144–1150. doi: 10.1016/s0006-291x(83)80262-9. [DOI] [PubMed] [Google Scholar]
- Woodbury R. G., Katunuma N., Kobayashi K., Titani K., Neurath H., Anderson W. F., Matthews B. W. Covalent structure of a group-specific protease from rat small intestine. Appendix: crystallographic data for a group specific protease from rat intestine. Biochemistry. 1978 Mar 7;17(5):811–819. doi: 10.1021/bi00598a010. [DOI] [PubMed] [Google Scholar]
- von Heijne G. A new method for predicting signal sequence cleavage sites. Nucleic Acids Res. 1986 Jun 11;14(11):4683–4690. doi: 10.1093/nar/14.11.4683. [DOI] [PMC free article] [PubMed] [Google Scholar]
- von Heijne G. Signal sequences. The limits of variation. J Mol Biol. 1985 Jul 5;184(1):99–105. doi: 10.1016/0022-2836(85)90046-4. [DOI] [PubMed] [Google Scholar]