Abstract
Tn7 transposition is abolished by the deletion of a 2.2kb HindIII fragment from the central region of the transposon. Transposition is restored when the fragment is present in trans. When this fragment is present in trans with wild-type Tn7, transposition frequencies are stimulated 10-100-fold. The DNA sequence of this fragment has been determined and found to contain one long open reading frame coding for a protein of molecular weight 61 187. We have visualised this protein using a DNA-directed prokaryotic transcription-translation system. This gene may fill a regulatory role in the mechanism of Tn7 transposition. When present in trans the 2.2kb HindIII fragment alleviates a transcriptional block of promoter activity detected in Tn7.
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Selected References
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- Barth P. T., Datta N., Hedges R. W., Grinter N. J. Transposition of a deoxyribonucleic acid sequence encoding trimethoprim and streptomycin resistances from R483 to other replicons. J Bacteriol. 1976 Mar;125(3):800–810. doi: 10.1128/jb.125.3.800-810.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Barth P. T., Grinter N. J. Map of plasmid RP4 derived by insertion of transposon C. J Mol Biol. 1977 Jul 5;113(3):455–474. doi: 10.1016/0022-2836(77)90233-9. [DOI] [PubMed] [Google Scholar]
- Brutlag D. L., Clayton J., Friedland P., Kedes L. H. SEQ: a nucleotide sequence analysis and recombination system. Nucleic Acids Res. 1982 Jan 11;10(1):279–294. doi: 10.1093/nar/10.1.279. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Caruso M., Shapiro J. A. Interactions of Tn7 and temperate phage F116L of Pseudomonas aeruginosa. Mol Gen Genet. 1982;188(2):292–298. doi: 10.1007/BF00332690. [DOI] [PubMed] [Google Scholar]
- Casadaban M. J., Chou J., Cohen S. N. In vitro gene fusions that join an enzymatically active beta-galactosidase segment to amino-terminal fragments of exogenous proteins: Escherichia coli plasmid vectors for the detection and cloning of translational initiation signals. J Bacteriol. 1980 Aug;143(2):971–980. doi: 10.1128/jb.143.2.971-980.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Chang A. C., Cohen S. N. Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid. J Bacteriol. 1978 Jun;134(3):1141–1156. doi: 10.1128/jb.134.3.1141-1156.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ely B. Transposition of Tn7 occurs at a single site on the Caulobacter crescentus chromosome. J Bacteriol. 1982 Aug;151(2):1056–1058. doi: 10.1128/jb.151.2.1056-1058.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fennewald M. A., Shapiro J. A. Transposition of Tn7 in Pseudomonas aeruginosa and isolation of alk::Tn7 mutations. J Bacteriol. 1979 Jul;139(1):264–269. doi: 10.1128/jb.139.1.264-269.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gosti-Testu F., Norris V., Brevet J. Restriction map of Tn7. Plasmid. 1983 Jul;10(1):96–99. doi: 10.1016/0147-619x(83)90061-6. [DOI] [PubMed] [Google Scholar]
- Holt G. R., Davis W. E., Ailor E. I., Warren A. H., Elyassi H. Massive airway hemorrhage after transtracheal aspiration. South Med J. 1978 Mar;71(3):325–327. doi: 10.1097/00007611-197803000-00031. [DOI] [PubMed] [Google Scholar]
- Kleckner N. Transposable elements in prokaryotes. Annu Rev Genet. 1981;15:341–404. doi: 10.1146/annurev.ge.15.120181.002013. [DOI] [PubMed] [Google Scholar]
- Lichtenstein C., Brenner S. Site-specific properties of Tn7 transposition into the E. coli chromosome. Mol Gen Genet. 1981;183(2):380–387. doi: 10.1007/BF00270644. [DOI] [PubMed] [Google Scholar]
- Lichtenstein C., Brenner S. Unique insertion site of Tn7 in the E. coli chromosome. Nature. 1982 Jun 17;297(5867):601–603. doi: 10.1038/297601a0. [DOI] [PubMed] [Google Scholar]
- Messing J., Crea R., Seeburg P. H. A system for shotgun DNA sequencing. Nucleic Acids Res. 1981 Jan 24;9(2):309–321. doi: 10.1093/nar/9.2.309. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Messing J., Vieira J. A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments. Gene. 1982 Oct;19(3):269–276. doi: 10.1016/0378-1119(82)90016-6. [DOI] [PubMed] [Google Scholar]
- Ouartsi A., Borowski D., Brevet J. Genetic analysis of Tn7 transposition. Mol Gen Genet. 1985;198(2):221–227. doi: 10.1007/BF00382999. [DOI] [PubMed] [Google Scholar]
- Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Smith G. M., Jones P. Effects of deletions in transposon Tn7 on its frequency of transposition. J Bacteriol. 1984 Mar;157(3):962–964. doi: 10.1128/jb.157.3.962-964.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Smith G. M., Shaw W. V. Comparison of three methods for the purification of the delta haemolysin of Staphylococcus aureus. J Gen Microbiol. 1981 Jun;124(2):365–374. doi: 10.1099/00221287-124-2-365. [DOI] [PubMed] [Google Scholar]
- Thomson J. A., Hendson M., Magnes R. M. Mutagenesis by insertion of drug resistance transposon Tn7 into a vibrio species. J Bacteriol. 1981 Oct;148(1):374–378. doi: 10.1128/jb.148.1.374-378.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Twigg A. J., Sherratt D. Trans-complementable copy-number mutants of plasmid ColE1. Nature. 1980 Jan 10;283(5743):216–218. doi: 10.1038/283216a0. [DOI] [PubMed] [Google Scholar]