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. 1986 Nov 11;14(21):8231–8245. doi: 10.1093/nar/14.21.8231

Insertion elements and transitions in cloned mouse mammary tumour virus DNA: further delineation of the poison sequences.

S Brookes, M Placzek, R Moore, M Dixon, C Dickson, G Peters
PMCID: PMC311856  PMID: 3024101

Abstract

The provirus of mouse mammary tumour virus (MMTV) is reputed to contain sequences within the viral gag gene that prevent or inhibit its propagation as a recombinant DNA clone in Escherichia coli. Here we report the successful isolation of several lambda and plasmid clones comprising the 5' virus-host DNA junction fragments from integrated MMTV proviruses in BR6 mice. Although the lambda clones appeared intact, almost all of the plasmids were found to contain the bacterial insertion sequences IS1 or IS2 within a small region of the gag gene. One nondisrupted clone was recovered which had undergone multiple G to A transitions, some of which created stop codons in gag. These results have provided more precise information as to the location of the poison sequences and are discussed in relation to possible explanations for the phenomenon.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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