Table 1.
Primer pairs used for PCR (5′→3′)
| Gene (expected size) |
External Primers | Internal Primers | Accession No. |
|---|---|---|---|
| GAPDH (493, 242 bp) |
GCTGAGTATGTCGTGGAGTCTA CATACTTGGCAGGTTTCTCCAG |
GTTTGTGATGGGTGTGAACCAC TGGATGCAGGGATGATGTTCTG |
NM_008084 |
| TRPV1 (486, 191 bp) |
GGGAAGAATAACTCACTGCCTGTG TGGGTCCTCGTTGATGATGC |
GGCGAGACTGTCAACAAGATTGC TCATCCACCCTGAAGCACCAC |
NM_001001445.1 |
| TRPA1 (495, 224 bp) |
CTTCCTGGATTACAACAATGCTCTG ATGTCCCCAACCGCCAAGC |
CAGTGGCAATGTGGAGCAATAG AAGGAAAGCAATGGGGTGC |
NM_177781.4 |
| TREK-1 (557, 229 bp) |
CACATGGAGAGATACAGACTGC GAGATGGGTGGAGCTTTCTTTG |
GAGATACAGACTGCTGGCATAG GTAGATGTAAGTACGGGCACAG |
NM_010607 |
| TREK-2 (438, 254 bp) |
CAGTGGCAACGCTATAGTTCTC CCACCTACACTACCTATCCCAT |
GTGATAGGTGGTGCACAGATAG GCCATTGGTTAAGGAGATAGCC |
NM_029911 |
| TRAAK (427, 109 bp) |
CAGTGAGAATCTGGCCTTCATC TTTGAGGCACAGTTGTGAGG |
ATTTGACCAAAGAGCCGTCC TTGGATGTGAAGAACCAGCC |
NM_008431 |