Abstract
The effects of the binding of the E. coli UvrA and UvrB proteins on the linking number (delta L) of superhelical DNA has been measured. The effects of cofactor ATP structure on UvrAB-nucleoprotein complex formation revealed that nucleotide binding, not hydrolysis, is sufficient to locally unwind the DNA helix of both ultraviolet light-damaged as well as undamaged DNAs. The extent of this unwinding is of the same order of magnitude as the nucleotide distances of the double incision sites generated by the UvrABC endonucleolytic reaction.
Full text
PDF














Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Beck D. J., Popoff S., Sancar A., Rupp W. D. Reactions of the UVRABC excision nuclease with DNA damaged by diamminedichloroplatinum(II). Nucleic Acids Res. 1985 Oct 25;13(20):7395–7412. doi: 10.1093/nar/13.20.7395. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bryant F. R., Taylor A. R., Lehman I. R. Interaction of the recA protein of Escherichia coli with single-stranded DNA. J Biol Chem. 1985 Jan 25;260(2):1196–1202. [PubMed] [Google Scholar]
- Caron P. R., Kushner S. R., Grossman L. Involvement of helicase II (uvrD gene product) and DNA polymerase I in excision mediated by the uvrABC protein complex. Proc Natl Acad Sci U S A. 1985 Aug;82(15):4925–4929. doi: 10.1073/pnas.82.15.4925. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ciarrocchi G., Pedrini A. M. Determination of pyrimidine dimer unwinding angle by measurement of DNA electrophoretic mobility. J Mol Biol. 1982 Feb 25;155(2):177–183. doi: 10.1016/0022-2836(82)90445-4. [DOI] [PubMed] [Google Scholar]
- Cleaver J. E., Bootsma D. Xeroderma pigmentosum: biochemical and genetic characteristics. Annu Rev Genet. 1975;9:19–38. doi: 10.1146/annurev.ge.09.120175.000315. [DOI] [PubMed] [Google Scholar]
- Depew D. E., Wang J. C. Conformational fluctuations of DNA helix. Proc Natl Acad Sci U S A. 1975 Nov;72(11):4275–4279. doi: 10.1073/pnas.72.11.4275. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Frederick C. A., Grable J., Melia M., Samudzi C., Jen-Jacobson L., Wang B. C., Greene P., Boyer H. W., Rosenberg J. M. Kinked DNA in crystalline complex with EcoRI endonuclease. Nature. 1984 May 24;309(5966):327–331. doi: 10.1038/309327a0. [DOI] [PubMed] [Google Scholar]
- Gamper H. B., Hearst J. E. A topological model for transcription based on unwinding angle analysis of E. coli RNA polymerase binary, initiation and ternary complexes. Cell. 1982 May;29(1):81–90. doi: 10.1016/0092-8674(82)90092-7. [DOI] [PubMed] [Google Scholar]
- Hines J. C., Ray D. S. Construction and characterization of new coliphage M13 cloning vectors. Gene. 1980 Nov;11(3-4):207–218. doi: 10.1016/0378-1119(80)90061-x. [DOI] [PubMed] [Google Scholar]
- Husain I., Van Houten B., Thomas D. C., Abdel-Monem M., Sancar A. Effect of DNA polymerase I and DNA helicase II on the turnover rate of UvrABC excision nuclease. Proc Natl Acad Sci U S A. 1985 Oct;82(20):6774–6778. doi: 10.1073/pnas.82.20.6774. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kolb A., Buc H. Is DNA unwound by the cyclic AMP receptor protein? Nucleic Acids Res. 1982 Jan 22;10(2):473–485. doi: 10.1093/nar/10.2.473. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pedrini A. M., Ciarrocchi G. Inhibition of Micrococcus luteus DNA topoisomerase I by UV photoproducts. Proc Natl Acad Sci U S A. 1983 Apr;80(7):1787–1791. doi: 10.1073/pnas.80.7.1787. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pulleyblank D. E., Shure M., Vinograd J. The quantitation of fluorescence by photography. Nucleic Acids Res. 1977;4(5):1409–1418. doi: 10.1093/nar/4.5.1409. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sancar A., Franklin K. A., Sancar G. B. Escherichia coli DNA photolyase stimulates uvrABC excision nuclease in vitro. Proc Natl Acad Sci U S A. 1984 Dec;81(23):7397–7401. doi: 10.1073/pnas.81.23.7397. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sancar A., Franklin K. A., Sancar G., Tang M. S. Repair of psoralen and acetylaminofluorene DNA adducts by ABC excinuclease. J Mol Biol. 1985 Aug 20;184(4):725–734. doi: 10.1016/0022-2836(85)90316-x. [DOI] [PubMed] [Google Scholar]
- Sancar A., Rupp W. D. A novel repair enzyme: UVRABC excision nuclease of Escherichia coli cuts a DNA strand on both sides of the damaged region. Cell. 1983 May;33(1):249–260. doi: 10.1016/0092-8674(83)90354-9. [DOI] [PubMed] [Google Scholar]
- Scovell W. M., Collart F. Unwinding of supercoiled DNA by cis- and trans-diamminedichloroplatinum(II): influence of the torsional strain on DNA unwinding. Nucleic Acids Res. 1985 Apr 25;13(8):2881–2895. doi: 10.1093/nar/13.8.2881. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Seeberg E., Steinum A. L., Nordenskjöld M., Söderhäll S., Jernström B. Strand-break formation in DNA modified by benzo[alpha]pyrene diolepoxide. Quantitative cleavage by Escherichia coli uvrABC endonuclease. Mutat Res. 1983 Jun;112(3):139–145. doi: 10.1016/0167-8817(83)90036-6. [DOI] [PubMed] [Google Scholar]
- Seeberg E., Steinum A. L. Purification and properties of the uvrA protein from Escherichia coli. Proc Natl Acad Sci U S A. 1982 Feb;79(4):988–992. doi: 10.1073/pnas.79.4.988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Shure M., Pulleyblank D. E., Vinograd J. The problems of eukaryotic and prokaryotic DNA packaging and in vivo conformation posed by superhelix density heterogeneity. Nucleic Acids Res. 1977;4(5):1183–1205. doi: 10.1093/nar/4.5.1183. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sugino A., Higgins N. P., Brown P. O., Peebles C. L., Cozzarelli N. R. Energy coupling in DNA gyrase and the mechanism of action of novobiocin. Proc Natl Acad Sci U S A. 1978 Oct;75(10):4838–4842. doi: 10.1073/pnas.75.10.4838. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Thomas D. C., Levy M., Sancar A. Amplification and purification of UvrA, UvrB, and UvrC proteins of Escherichia coli. J Biol Chem. 1985 Aug 15;260(17):9875–9883. [PubMed] [Google Scholar]
- Wang J. C., Jacobsen J. H., Saucier J. M. Physiochemical studies on interactions between DNA and RNA polymerase. Unwinding of the DNA helix by Escherichia coli RNA polymerase. Nucleic Acids Res. 1977;4(5):1225–1241. doi: 10.1093/nar/4.5.1225. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wiesehahn G., Hearst J. E. DNA unwinding induced by photoaddition of psoralen derivatives and determination of dark-binding equilibrium constants by gel electrophoresis. Proc Natl Acad Sci U S A. 1978 Jun;75(6):2703–2707. doi: 10.1073/pnas.75.6.2703. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wu H. M., Crothers D. M. The locus of sequence-directed and protein-induced DNA bending. Nature. 1984 Apr 5;308(5959):509–513. doi: 10.1038/308509a0. [DOI] [PubMed] [Google Scholar]
- Yeung A. T., Mattes W. B., Grossman L. Protein complexes formed during the incision reaction catalyzed by the Escherichia coli UvrABC endonuclease. Nucleic Acids Res. 1986 Mar 25;14(6):2567–2582. doi: 10.1093/nar/14.6.2567. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Yeung A. T., Mattes W. B., Oh E. Y., Grossman L. Enzymatic properties of purified Escherichia coli uvrABC proteins. Proc Natl Acad Sci U S A. 1983 Oct;80(20):6157–6161. doi: 10.1073/pnas.80.20.6157. [DOI] [PMC free article] [PubMed] [Google Scholar]