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. 1986 Nov 11;14(21):8595–8603. doi: 10.1093/nar/14.21.8595

Sequencing and identification of a cDNA clone for tomato polygalacturonase.

D Grierson, G A Tucker, J Keen, J Ray, C R Bird, W Schuch
PMCID: PMC311879  PMID: 3786135

Abstract

The 2a isoenzyme of tomato polygalacturonase was purified from ripe fruit and characterised. The N-terminal amino acid sequence of the protein was determined in order to identify polygalacturonase cDNA clones. The nucleotide sequence of a ripening-related cDNA (pTOM 6) was determined and found to encode the N-terminal sequence of mature polygalacturonase 2a. The complete open reading frame encodes a polypeptide of molecular weight 50,051, including a putative pre-sequence of 71 amino acids.

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Selected References

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