Skip to main content
NCBI home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1986 Nov 25;14(22):9051–9061. doi: 10.1093/nar/14.22.9051

Nucleotide sequence of the gene encoding the matrix protein of Newcastle disease virus.

P Chambers, N S Millar, S G Platt, P T Emmerson
PMCID: PMC311928  PMID: 3786143

Abstract

The nucleotide sequence of the gene encoding the matrix (M) protein of the Beaudette C strain of Newcastle disease virus (NDV) has been determined from overlapping cDNA clones. Control sequences typical of paramyxovirus mRNA start and polyadenylation signals have been identified. Assuming that the M gene starts and finishes at these sequences, the M gene is 1241 nucleotides long and encodes one long open reading frame of 364 amino acids, corresponding to a polypeptide of molecular weight 39605, in good agreement with estimates from SDS gels. The M protein has an amino acid sequence that is both hydrophobic and highly basic. The NDV M protein has sequence homologies to the M proteins of Sendai, measles, canine distemper and respiratory syncytial viruses.

Full text

PDF
9051

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bellini W. J., Englund G., Richardson C. D., Rozenblatt S., Lazzarini R. A. Matrix genes of measles virus and canine distemper virus: cloning, nucleotide sequences, and deduced amino acid sequences. J Virol. 1986 May;58(2):408–416. doi: 10.1128/jvi.58.2.408-416.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Blumberg B. M., Rose K., Simona M. G., Roux L., Giorgi C., Kolakofsky D. Analysis of the Sendai virus M gene and protein. J Virol. 1984 Nov;52(2):656–663. doi: 10.1128/jvi.52.2.656-663.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bohn W., Rutter G., Hohenberg H., Mannweiler K., Nobis P. Involvement of actin filaments in budding of measles virus: studies on cytoskeletons of infected cells. Virology. 1986 Feb;149(1):91–106. doi: 10.1016/0042-6822(86)90090-5. [DOI] [PubMed] [Google Scholar]
  4. Büechi M., Bächi T. Microscopy of internal structures of Sendai virus associated with the cytoplasmic surface of host membranes. Virology. 1982 Jul 30;120(2):349–359. doi: 10.1016/0042-6822(82)90036-8. [DOI] [PubMed] [Google Scholar]
  5. Caldwell S. E., Lyles D. S. Dissociation of newly synthesized Sendai viral proteins from the cytoplasmic surface of isolated plasma membranes of infected cells. J Virol. 1986 Feb;57(2):678–683. doi: 10.1128/jvi.57.2.678-683.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Caldwell S. E., Lyles D. S. Interaction of Sendai virus proteins with the cytoplasmic surface of erythrocyte membranes following viral envelope fusion. J Biol Chem. 1981 May 25;256(10):4838–4842. [PubMed] [Google Scholar]
  7. Chambers P., Millar N. S., Bingham R. W., Emmerson P. T. Molecular cloning of complementary DNA to Newcastle disease virus, and nucleotide sequence analysis of the junction between the genes encoding the haemagglutinin-neuraminidase and the large protein. J Gen Virol. 1986 Mar;67(Pt 3):475–486. doi: 10.1099/0022-1317-67-3-475. [DOI] [PubMed] [Google Scholar]
  8. Chambers P., Samson A. C. A new structural protein for Newcastle disease virus. J Gen Virol. 1980 Sep;50(1):155–166. doi: 10.1099/0022-1317-50-1-155. [DOI] [PubMed] [Google Scholar]
  9. Garnier J., Osguthorpe D. J., Robson B. Analysis of the accuracy and implications of simple methods for predicting the secondary structure of globular proteins. J Mol Biol. 1978 Mar 25;120(1):97–120. doi: 10.1016/0022-2836(78)90297-8. [DOI] [PubMed] [Google Scholar]
  10. Giuffre R. M., Tovell D. R., Kay C. M., Tyrrell D. L. Evidence for an interaction between the membrane protein of a paramyxovirus and actin. J Virol. 1982 Jun;42(3):963–968. doi: 10.1128/jvi.42.3.963-968.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Gregoriades A., Frangione B. Insertion of influenza M protein into the viral lipid bilayer and localization of site of insertion. J Virol. 1981 Oct;40(1):323–328. doi: 10.1128/jvi.40.1.323-328.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Gupta K. C., Kingsbury D. W. Complete sequences of the intergenic and mRNA start signals in the Sendai virus genome: homologies with the genome of vesicular stomatitis virus. Nucleic Acids Res. 1984 May 11;12(9):3829–3841. doi: 10.1093/nar/12.9.3829. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Hewitt J. A., Nermut M. V. A morphological study of the M-protein of Sendai virus. J Gen Virol. 1977 Jan;34(1):127–136. doi: 10.1099/0022-1317-34-1-127. [DOI] [PubMed] [Google Scholar]
  14. Ishida N., Taira H., Omata T., Mizumoto K., Hattori S., Iwasaki K., Kawakita M. Sequence of 2,617 nucleotides from the 3' end of Newcastle disease virus genome RNA and the predicted amino acid sequence of viral NP protein. Nucleic Acids Res. 1986 Aug 26;14(16):6551–6564. doi: 10.1093/nar/14.16.6551. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Kolakofsky D., Boy de la Tour E., Delius H. Molecular weight determination of Sendai and Newcastle disease virus RNA. J Virol. 1974 Feb;13(2):261–268. doi: 10.1128/jvi.13.2.261-268.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Lamb R. A., Choppin P. W. The synthesis of Sendai virus polypeptides in infected cells. III. Phosphorylation of polypeptides. Virology. 1977 Sep;81(2):382–397. doi: 10.1016/0042-6822(77)90154-4. [DOI] [PubMed] [Google Scholar]
  17. Li J. K., Miyakawa T., Fox C. F. Protein organization in Newcastle disease virus as revealed by perturbant treatment. J Virol. 1980 Apr;34(1):268–271. doi: 10.1128/jvi.34.1.268-271.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Markwell M. A., Fox C. F. Protein-protein interactions within paramyxoviruses identified by native disulfide bonding or reversible chemical cross-linking. J Virol. 1980 Jan;33(1):152–166. doi: 10.1128/jvi.33.1.152-166.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Marx P. A., Portner A., Kingsbury D. W. Sendai virion transcriptase complex: polyeptide composition and inhibition by virion envelope proteins. J Virol. 1974 Jan;13(1):107–112. doi: 10.1128/jvi.13.1.107-112.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. McCauley J. W., Mahy B. W., Inglis S. C. Nucleotide sequence of fowl plague virus RNA segment 7. J Gen Virol. 1982 Jan;58(Pt 1):211–215. doi: 10.1099/0022-1317-58-1-211. [DOI] [PubMed] [Google Scholar]
  21. Millar N. S., Chambers P., Emmerson P. T. Nucleotide sequence analysis of the haemagglutinin-neuraminidase gene of Newcastle disease virus. J Gen Virol. 1986 Sep;67(Pt 9):1917–1927. doi: 10.1099/0022-1317-67-9-1917. [DOI] [PubMed] [Google Scholar]
  22. Nagai Y., Klenk H. D., Rott R. Proteolytic cleavage of the viral glycoproteins and its significance for the virulence of Newcastle disease virus. Virology. 1976 Jul 15;72(2):494–508. doi: 10.1016/0042-6822(76)90178-1. [DOI] [PubMed] [Google Scholar]
  23. Queen C., Korn L. J. A comprehensive sequence analysis program for the IBM personal computer. Nucleic Acids Res. 1984 Jan 11;12(1 Pt 2):581–599. doi: 10.1093/nar/12.1part2.581. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Rose J. K., Gallione C. J. Nucleotide sequences of the mRNA's encoding the vesicular stomatitis virus G and M proteins determined from cDNA clones containing the complete coding regions. J Virol. 1981 Aug;39(2):519–528. doi: 10.1128/jvi.39.2.519-528.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Roux L., Kolakofsky D. Protein kinase associated with Sendai virions. J Virol. 1974 Feb;13(2):545–547. doi: 10.1128/jvi.13.2.545-547.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Satake M., Venkatesan S. Nucleotide sequence of the gene encoding respiratory syncytial virus matrix protein. J Virol. 1984 Apr;50(1):92–99. doi: 10.1128/jvi.50.1.92-99.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Scheid A., Choppin P. W. Isolation and purification of the envelope proteins of Newcastle disease virus. J Virol. 1973 Feb;11(2):263–271. doi: 10.1128/jvi.11.2.263-271.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Shimizu K., Isida N. The smallest protein of Sendi virus: its candidate function of binding nucleocaspsid to envelope. Virology. 1975 Oct;67(2):427–437. [PubMed] [Google Scholar]
  29. Smith G. W., Hightower L. E. Identification of the P proteins and other disulfide-linked and phosphorylated proteins of Newcastle disease virus. J Virol. 1981 Jan;37(1):256–267. doi: 10.1128/jvi.37.1.256-267.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Staden R. Automation of the computer handling of gel reading data produced by the shotgun method of DNA sequencing. Nucleic Acids Res. 1982 Aug 11;10(15):4731–4751. doi: 10.1093/nar/10.15.4731. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Staden R. Graphic methods to determine the function of nucleic acid sequences. Nucleic Acids Res. 1984 Jan 11;12(1 Pt 2):521–538. doi: 10.1093/nar/12.1part2.521. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Staden R. The current status and portability of our sequence handling software. Nucleic Acids Res. 1986 Jan 10;14(1):217–231. doi: 10.1093/nar/14.1.217. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Waterson A. P., Pennington T. H., Allan W. H. Virulence in Newcastle disease virus. A preliminary study. Br Med Bull. 1967 May;23(2):138–143. doi: 10.1093/oxfordjournals.bmb.a070534. [DOI] [PubMed] [Google Scholar]
  34. Wiener J. R., Pal R., Barenholz Y., Wagner R. R. Influence of the peripheral matrix protein of vesicular stomatitis virus on the membrane dynamics of mixed phospholipid vesicles: fluorescence studies. Biochemistry. 1983 Apr 26;22(9):2162–2170. doi: 10.1021/bi00278a017. [DOI] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES