Figure 6.
Effect of leptomycin B treatment of TRIM5α-expressing cells on retrovirus restriction. Cf2Th cells stably expressing TRIM5αrh or transduced with the empty vector LPCX were challenged with increasing amounts of HIV-1-GFP in the presence of 5 ng/ml of LMB or DMSO (A). Similarly, Cf2Th cells stably expressing TRIM5αhu were challenged with increasing amounts of N-MLV-GFP in the presence of 5 ng/ml of LMB or DMSO (B). TE671 cells, which naturally express TRIM5αhu, were challenged with B-MLV-GFP and N-MLV-GFP in the presence of the indicated concentration of LMB or the DMSO control (C). The x-axis indicates the volume of a stock of recombinant GFP-expressing virus added to the target cells. Forty-eight hours after infection, the percentage of infected cells was measured by counting the GFP-positive cells using a flow cytometer. Similar results were obtained in three independent experiments.