Skip to main content
NCBI home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1992 Mar 11;20(5):1011–1016. doi: 10.1093/nar/20.5.1011

Yeast ribosomal proteins: XIII. Saccharomyces cerevisiae YL8A gene, interrupted with two introns, encodes a homolog of mammalian L7.

K Mizuta 1, T Hashimoto 1, E Otaka 1
PMCID: PMC312084  PMID: 1549461

Abstract

We isolated and sequenced a gene, YL8A, encoding ribosomal protein YL8 of Saccharomyces cerevisiae. It is one of the two duplicated genes encoding YL8 and is located on chromosome VII while the other is on chromosome XVI. The haploid strains carrying disrupted YL8A grew more slowly than the parent strain. The open reading frame is interrupted with two introns. The predicted amino acid sequence reveals that yeast YL8 is a homolog of mammalian ribosomal protein L7, E.coli L30 and others.

Full text

PDF
1011

Images in this article

1013Image
on p.1013
1013Image
on p.1013
1013Image
on p.1013
1014Image
on p.1014

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Auer J., Spicker G., Böck A. Organization and structure of the Methanococcus transcriptional unit homologous to the Escherichia coli "spectinomycin operon". Implications for the evolutionary relationship of 70 S and 80 S ribosomes. J Mol Biol. 1989 Sep 5;209(1):21–36. doi: 10.1016/0022-2836(89)90167-8. [DOI] [PubMed] [Google Scholar]
  2. Cerretti D. P., Dean D., Davis G. R., Bedwell D. M., Nomura M. The spc ribosomal protein operon of Escherichia coli: sequence and cotranscription of the ribosomal protein genes and a protein export gene. Nucleic Acids Res. 1983 May 11;11(9):2599–2616. doi: 10.1093/nar/11.9.2599. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Dabeva M. D., Post-Beittenmiller M. A., Warner J. R. Autogenous regulation of splicing of the transcript of a yeast ribosomal protein gene. Proc Natl Acad Sci U S A. 1986 Aug;83(16):5854–5857. doi: 10.1073/pnas.83.16.5854. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Damagnez V., de Recondo A. M., Baldacci G. Identification of a gene encoding the predicted ribosomal protein L7b divergently transcribed from POL1 in fission yeast Schizosaccharomyces pombe. Nucleic Acids Res. 1991 Mar 11;19(5):1099–1104. doi: 10.1093/nar/19.5.1099. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Fink G. R. Pseudogenes in yeast? Cell. 1987 Apr 10;49(1):5–6. doi: 10.1016/0092-8674(87)90746-x. [DOI] [PubMed] [Google Scholar]
  6. Giri L., Hill W. E., Wittmann H. G., Wittmann-Liebold B. Ribosomal proteins: their structure and spatial arrangement in prokaryotic ribosomes. Adv Protein Chem. 1984;36:1–78. doi: 10.1016/s0065-3233(08)60295-8. [DOI] [PubMed] [Google Scholar]
  7. Hanahan D. Studies on transformation of Escherichia coli with plasmids. J Mol Biol. 1983 Jun 5;166(4):557–580. doi: 10.1016/s0022-2836(83)80284-8. [DOI] [PubMed] [Google Scholar]
  8. Hatakeyama T., Kaufmann F., Schroeter B., Hatakeyama T. Primary structures of five ribosomal proteins from the archaebacterium Halobacterium marismortui and their structural relationships to eubacterial and eukaryotic ribosomal proteins. Eur J Biochem. 1989 Nov 20;185(3):685–693. doi: 10.1111/j.1432-1033.1989.tb15166.x. [DOI] [PubMed] [Google Scholar]
  9. Ito H., Fukuda Y., Murata K., Kimura A. Transformation of intact yeast cells treated with alkali cations. J Bacteriol. 1983 Jan;153(1):163–168. doi: 10.1128/jb.153.1.163-168.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Itoh T., Higo K., Otaka E. Isolation and characterization of twenty-three ribosomal proteins from large subunits of yeast. Biochemistry. 1979 Dec 25;18(26):5787–5791. doi: 10.1021/bi00593a007. [DOI] [PubMed] [Google Scholar]
  11. Itoh T., Otaka E., Matsui K. A. Primary structures of ribosomal protein YS25 from Saccharomyces cerevisiae and its counterparts from Schizosaccharomyces pombe and rat liver. Biochemistry. 1985 Dec 3;24(25):7418–7423. doi: 10.1021/bi00346a058. [DOI] [PubMed] [Google Scholar]
  12. Iwabe N., Kuma K., Kishino H., Hasegawa M., Miyata T. Compartmentalized isozyme genes and the origin of introns. J Mol Evol. 1990 Sep;31(3):205–210. doi: 10.1007/BF02109497. [DOI] [PubMed] [Google Scholar]
  13. Jensen R., Sprague G. F., Jr, Herskowitz I. Regulation of yeast mating-type interconversion: feedback control of HO gene expression by the mating-type locus. Proc Natl Acad Sci U S A. 1983 May;80(10):3035–3039. doi: 10.1073/pnas.80.10.3035. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Kang W., Matsushita Y., Grohmann L., Graack H. R., Kitakawa M., Isono K. Cloning and analysis of the nuclear gene for YmL33, a protein of the large subunit of the mitochondrial ribosome in Saccharomyces cerevisiae. J Bacteriol. 1991 Jul;173(13):4013–4020. doi: 10.1128/jb.173.13.4013-4020.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Kimura M. Proteins of the Bacillus stearothermophilus ribosome. The amino acid sequences of proteins S5 and L30. J Biol Chem. 1984 Jan 25;259(2):1051–1055. [PubMed] [Google Scholar]
  16. Kubota Y., Nishikawa K., Takahashi S., Ooi T. Correspondence of homologies in amino acid sequence and tertiary structure of protein molecules. Biochim Biophys Acta. 1982 Feb 18;701(2):242–252. doi: 10.1016/0167-4838(82)90120-0. [DOI] [PubMed] [Google Scholar]
  17. Lin A., Chan Y. L., McNally J., Peleg D., Meyuhas O., Wool I. G. The primary structure of rat ribosomal protein L7. The presence near the amino terminus of L7 of five tandem repeats of a sequence of 12 amino acids. J Biol Chem. 1987 Sep 15;262(26):12665–12671. [PubMed] [Google Scholar]
  18. Lipman D. J., Pearson W. R. Rapid and sensitive protein similarity searches. Science. 1985 Mar 22;227(4693):1435–1441. doi: 10.1126/science.2983426. [DOI] [PubMed] [Google Scholar]
  19. Meyuhas O., Klein A. The mouse ribosomal protein L7 gene. Its primary structure and functional analysis of the promoter region. J Biol Chem. 1990 Jul 15;265(20):11465–11473. [PubMed] [Google Scholar]
  20. Miller A. M. The yeast MATa1 gene contains two introns. EMBO J. 1984 May;3(5):1061–1065. doi: 10.1002/j.1460-2075.1984.tb01927.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Mizuta K., Hashimoto T., Suzuki K., Otaka E. Yeast ribosomal proteins: XII. YS11 of Saccharomyces cerevisiae is a homologue to E. coli S4 according to the gene analysis. Nucleic Acids Res. 1991 May 25;19(10):2603–2608. doi: 10.1093/nar/19.10.2603. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Murray J. M., Watts F. Z. Isolation of a Schizosaccharomyces pombe homologue to the rat ribosomal protein, L7. Nucleic Acids Res. 1990 Aug 11;18(15):4590–4590. doi: 10.1093/nar/18.15.4590. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Otaka E., Higo K., Itoh T. Yeast ribosomal proteins: VII. Cytoplasmic ribosomal proteins from Schizosaccharomyces pombe. Mol Gen Genet. 1983;191(3):519–524. doi: 10.1007/BF00425772. [DOI] [PubMed] [Google Scholar]
  24. Otaka E., Higo K., Osawa S. Isolation of seventeen proteins and amino-terminal amino acid sequences of eight proteins from cytoplasmic ribosomes of yeast. Biochemistry. 1982 Sep 14;21(19):4545–4550. doi: 10.1021/bi00262a005. [DOI] [PubMed] [Google Scholar]
  25. Otaka E., Ooi T., Kumazaki T., Itoh T. Examination of protein sequence homologies: I. Eleven Escherichia coli L7/L12-type ribosomal "A" protein sequences from eubacteria and chloroplast. J Mol Evol. 1984;21(4):339–345. doi: 10.1007/BF02115652. [DOI] [PubMed] [Google Scholar]
  26. Otaka E., Suzuki K., Hashimoto T. Examination of protein sequence homologies. VII. The complementary molecular coevolution of ribosomal proteins equivalent to Escherichia coli L7/L12 and L10. Protein Seq Data Anal. 1990 Mar;3(1):11–19. [PubMed] [Google Scholar]
  27. Quigley F., Martin W. F., Cerff R. Intron conservation across the prokaryote-eukaryote boundary: structure of the nuclear gene for chloroplast glyceraldehyde-3-phosphate dehydrogenase from maize. Proc Natl Acad Sci U S A. 1988 Apr;85(8):2672–2676. doi: 10.1073/pnas.85.8.2672. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Suzuki K., Hashimoto T., Otaka E. Yeast ribosomal proteins: XI. Molecular analysis of two genes encoding YL41, an extremely small and basic ribosomal protein, from Saccharomyces cerevisiae. Curr Genet. 1990 Mar;17(3):185–190. doi: 10.1007/BF00312608. [DOI] [PubMed] [Google Scholar]
  30. Suzuki K., Otaka E. Cloning and nucleotide sequence of the gene encoding yeast ribosomal protein YS25. Nucleic Acids Res. 1988 Jul 11;16(13):6223–6223. doi: 10.1093/nar/16.13.6223. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Szymkowski D. E., Kelly B., Deering R. A. A Dictyostelium discoideum cDNA coding for a protein with homology to the rat ribosomal protein L7. Nucleic Acids Res. 1989 Jul 11;17(13):5393–5393. doi: 10.1093/nar/17.13.5393. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Vincent A., Petes T. D. Mitotic and meiotic gene conversion of Ty elements and other insertions in Saccharomyces cerevisiae. Genetics. 1989 Aug;122(4):759–772. doi: 10.1093/genetics/122.4.759. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Warner J. R. Synthesis of ribosomes in Saccharomyces cerevisiae. Microbiol Rev. 1989 Jun;53(2):256–271. doi: 10.1128/mr.53.2.256-271.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Woolford J. L., Jr Nuclear pre-mRNA splicing in yeast. Yeast. 1989 Nov-Dec;5(6):439–457. doi: 10.1002/yea.320050604. [DOI] [PubMed] [Google Scholar]
  35. Yanisch-Perron C., Vieira J., Messing J. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene. 1985;33(1):103–119. doi: 10.1016/0378-1119(85)90120-9. [DOI] [PubMed] [Google Scholar]
  36. Zaret K. S., Sherman F. DNA sequence required for efficient transcription termination in yeast. Cell. 1982 Mar;28(3):563–573. doi: 10.1016/0092-8674(82)90211-2. [DOI] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES