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. 1992 Mar 11;20(5):1109–1112. doi: 10.1093/nar/20.5.1109

Conformational transitions of poly(dA-bromo5dU) and poly(dA-iodo5dU) in solution.

M Vorlícková 1, J Chládková 1, J Kypr 1
PMCID: PMC312098  PMID: 1549473

Abstract

Extensive circular dichroism studies have been conducted with the title polynucleotides under various solution conditions. The studies provided the following information: (i) The halogen atoms in place of thymine methyl hinder the isomerization into X-DNA. (ii) The brominated but not iodinated polynucleotide isomerizes into Z-DNA in concentrated NaCl+NiCl2. The transition takes place at lower NiCl2 concentrations than with poly(dA-dT). (iii) The iodinated polynucleotide forms an unusual conformation in aqueous solution in which it is very stable. It isomerizes from this conformer into the usual B-type double helix in concentrated ethanol solutions. The isomerization is a two-state cooperative process. (iv) Both title polynucleotides undergo still another two-state cooperative transition in trifluorethanol solutions presumably into A-DNA showing a rather unusual circular dichroism spectrum.

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Selected References

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