Skip to main content
NCBI home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1992 Mar 25;20(6):1309–1314. doi: 10.1093/nar/20.6.1309

Structural change of a particular H2B histone gene possibly results in differences in its transcriptional regulation in different chicken tissues.

T Nakayama 1, Y Setoguchi 1
PMCID: PMC312175  PMID: 1561087

Abstract

The chicken H2B histone gene family consists of eight highly homologous members (H2B-I to H2B-VIII) belonging to two major histone gene clusters. Seven of these genes have been sequenced and shown to encode three different H2B protein variants. Northern analysis with a probe, which mainly consists of the 5'-flanking region containing the sequence for the mRNA leader of H2B-V encoding a particular H2B protein variant, revealed that the mRNA level transcribed from this particular gene was higher in the kidney than in the oviduct and lung. To elucidate whether the structure of the H2B gene differs in the three different tissues, we analyzed DNAs from the oviduct, lung, and kidney. On Southern analysis, various H2B gene-specific probes hybridized with two particular H2B genes (H2B-IV and H2B-V), which are located in close proximity within a 12 kb EcoRI fragment, from the oviduct and lung with an intensity of about one quarter of that from the kidney. These findings suggest that some difference of DNA structure of the H2B-V gene may result in its relatively higher expression in the kidney.

Full text

PDF
1309

Images in this article

1311Image
on p.1311
1312Image
on p.1312
1312Image
on p.1312

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Blin N., Stafford D. W. A general method for isolation of high molecular weight DNA from eukaryotes. Nucleic Acids Res. 1976 Sep;3(9):2303–2308. doi: 10.1093/nar/3.9.2303. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Busslinger M., Schümperli D., Birnstiel M. L. Regulation of histone gene expression. Cold Spring Harb Symp Quant Biol. 1985;50:665–670. doi: 10.1101/sqb.1985.050.01.081. [DOI] [PubMed] [Google Scholar]
  3. Coles L. S., Robins A. J., Madley L. K., Wells J. R. Characterization of the chicken histone H1 gene complement. Generation of a complete set of vertebrate H1 protein sequences. J Biol Chem. 1987 Jul 15;262(20):9656–9663. [PubMed] [Google Scholar]
  4. D'Andrea R. J., Coles L. S., Lesnikowski C., Tabe L., Wells J. R. Chromosomal organization of chicken histone genes: preferred associations and inverted duplications. Mol Cell Biol. 1985 Nov;5(11):3108–3115. doi: 10.1128/mcb.5.11.3108. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Fletcher C., Heintz N., Roeder R. G. Purification and characterization of OTF-1, a transcription factor regulating cell cycle expression of a human histone H2b gene. Cell. 1987 Dec 4;51(5):773–781. doi: 10.1016/0092-8674(87)90100-0. [DOI] [PubMed] [Google Scholar]
  6. Grandy D. K., Dodgson J. B. Structure and organization of the chicken H2B histone gene family. Nucleic Acids Res. 1987 Feb 11;15(3):1063–1080. doi: 10.1093/nar/15.3.1063. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hentschel C. C., Birnstiel M. L. The organization and expression of histone gene families. Cell. 1981 Aug;25(2):301–313. doi: 10.1016/0092-8674(81)90048-9. [DOI] [PubMed] [Google Scholar]
  8. Huang H. C., Cole R. D. The distribution of H1 histone is nonuniform in chromatin and correlates with different degrees of condensation. J Biol Chem. 1984 Nov 25;259(22):14237–14242. [PubMed] [Google Scholar]
  9. Huh N. E., Hwang I. W., Lim K., You K. H., Chae C. B. Presence of a bi-directional S phase-specific transcription regulatory element in the promoter shared by testis-specific TH2A and TH2B histone genes. Nucleic Acids Res. 1991 Jan 11;19(1):93–98. doi: 10.1093/nar/19.1.93. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Nakayama T. Nucleotide sequences of two members of the chicken H3 histone-encoding gene family. Gene. 1991 Jun 30;102(2):289–290. doi: 10.1016/0378-1119(91)90092-p. [DOI] [PubMed] [Google Scholar]
  11. Nakayama T., Setoguchi Y. Nucleotide sequence of a member of the chicken H2B histone-encoding gene family. Gene. 1991 Feb 15;98(2):299–300. doi: 10.1016/0378-1119(91)90190-m. [DOI] [PubMed] [Google Scholar]
  12. Nakayama T., Takechi S., Ohshige T., Kondo K., Yamamoto K. Nucleotide sequences of two members of the chicken H4 histone-encoding gene family. Gene. 1991 Dec 15;108(2):311–312. doi: 10.1016/0378-1119(91)90452-h. [DOI] [PubMed] [Google Scholar]
  13. Ruiz-Carrillo A., Affolter M., Renaud J. Genomic organization of the genes coding for the six main histones of the chicken: complete sequence of the H5 gene. J Mol Biol. 1983 Nov 15;170(4):843–859. doi: 10.1016/s0022-2836(83)80191-0. [DOI] [PubMed] [Google Scholar]
  14. Setoguchi Y., Nakayama T. Nucleotide sequences of new members (H3-IV and H3-V) of the chicken H3 histone-encoding gene family. Nucleic Acids Res. 1991 Nov 25;19(22):6327–6327. doi: 10.1093/nar/19.22.6327. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Sharma A., Bos T. J., Pekkala-Flagan A., Vogt P. K., Lee A. S. Interaction of cellular factors related to the Jun oncoprotein with the promoter of a replication-dependent hamster histone H3.2 gene. Proc Natl Acad Sci U S A. 1989 Jan;86(2):491–495. doi: 10.1073/pnas.86.2.491. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  17. Sturm R. A., Dalton S., Wells J. R. Conservation of histone H2A/H2B intergene regions: a role for the H2B specific element in divergent transcription. Nucleic Acids Res. 1988 Sep 12;16(17):8571–8586. doi: 10.1093/nar/16.17.8571. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Sugarman B. J., Dodgson J. B., Engel J. D. Genomic organization, DNA sequence, and expression of chicken embryonic histone genes. J Biol Chem. 1983 Jul 25;258(14):9005–9016. [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES