Abstract
Intestinal epithelial cells serve as mechanical barriers and active components of the mucosal immune system. These cells migrate from the crypt to the tip of the villus, where different stimuli can differentially affect their survival. Here we investigated, using in vitro and in vivo strategies, the role of galectin-1 (Gal-1), an evolutionarily conserved glycan-binding protein, in modulating the survival of human and mouse enterocytes. Both Gal-1 and its specific glyco-receptors were broadly expressed in small bowel enterocytes. Exogenous Gal-1 reduced the viability of enterocytes through apoptotic mechanisms involving activation of both caspase and mitochondrial pathways. Consistent with these findings, apoptotic cells were mainly detected at the tip of the villi, following administration of Gal-1. Moreover, Gal-1-deficient (Lgals1−/−) mice showed longer villi compared with their wild-type counterparts in vivo. In an experimental model of starvation, fasted wild-type mice displayed reduced villi and lower intestinal weight compared with Lgals1−/− mutant mice, an effect reflected by changes in the frequency of enterocyte apoptosis. Of note, human small bowel enterocytes were also prone to this pro-apoptotic effect. Thus, Gal-1 is broadly expressed in mucosal tissue and influences the viability of human and mouse enterocytes, an effect which might influence the migration of these cells from the crypt, the integrity of the villus and the epithelial barrier function.
Keywords: galectin-1, small bowel, enterocytes, apoptosis, mucosa
The intestinal mucosa is continuously challenged by a variety of antigens from the diet and from commensal or pathogenic microorganisms, which should be carefully discriminated in order to either mount an active immune response or to promote immune tolerance.1 Epithelial cells, which are critical in this regulatory pathway, differentiate from stem cells located in the crypts and undergo a highly regulated maturation program as they migrate along the crypt-villus axis, forming a dynamic renewing tissue that maintains the integrity of the intestinal epithelium.2 As enterocytes migrate upwards along the axis, they acquire a mature absorptive phenotype, and are finally eliminated by apoptosis at the top of the villus. Several signaling cascades control cellular proliferation, differentiation, migration and apoptosis of enterocytes.3 Furthermore, different metabolites (iron, vitamins, amino acids, carbohydrates and lipids) and relevant enzymes including acyl-CoA synthetase serve as regulatory mediators that contribute to enterocyte renewal in the human intestine.3 However, the cellular and molecular mechanisms underlying the regulation of apoptosis of senescent enterocytes and maintenance of mucosal homeostasis, are still poorly understood.
Galectin-1 (Gal-1), an evolutionarily conserved β-galactoside-binding protein, has key roles in a variety of physiologic and pathologic processes.4, 5 These functions include suppression of T-cell responses through selective induction of TH1 and TH17 cell apoptosis4, 5 and activation of tolerogenic circuits on dendritic cells.6 These glycosylation-dependent functions account for the capacity of this lectin to dampen inflammation in autoimmune, chronic and acute inflammatory disorders,4, 7, 8, 9 and to favor immune escape of certain types of cancer.10, 11
Gal-1 is expressed in different portions of the human and mouse gastrointestinal tract,12, 13, 14 and similar to other members of the galectin family, has been implicated in different intestinal disorders.7, 14, 15, 16, 17 Gal-1 expression is downregulated in a mouse model of 2,4,6-trinitrobenzene sulfonic acid (TNBS)-induced colits and its therapeutic administration contributes to inhibition of TH1 and pro-inflammatory cytokines (TNF, IL-1β, IL-12 and IFN-γ), and remission of the clinical and histopathologic signs of inflammatory bowel disease.7 On the other hand, overexpression of Gal-1 has been linked to the progression of human colon carcinomas.12, 16
The present study was aimed at elucidating the role of Gal-1 in the intestinal epithelial cell compartment. We found that Gal-1 binds to human and mouse enterocytes where it signals apoptosis through a caspase-dependent mechanism, leading to depolarization of the mitochondrial membrane and caspase-3 activation. Accordingly, mice lacking Gal-1 (Lgals1−/−) have longer duodenal villi and exhibit a more pronounced deleterious response upon starvation compared with wild-type mice, suggesting that Gal-1 may be involved in overall regulation of enterocyte turnover in the villus, thus contributing to small bowel homeostasis.
Results
Co-expression of Gal-1 and Gal-1-binding sites in mouse small intestine correlates with a permissive ‘glycosylation signature'
To understand the role of Gal-1-glycan interactions in mucosal microenvironments, we first assessed the expression of Gal-1 and specific glycan structures in mouse small intestine. Gal-1 expression was evaluated in intestinal cells at the mRNA and protein levels by qRT-PCR and immunoblot analysis, respectively (Figures 1a and b). Duodenal mouse cells showed considerable expression of this glycan-binding protein (0.8 μg/mg protein or 3 μg/g wet intestinal tissue). Gal-1 was abundant within the lamina propria and muscularis mucosa (Figure 1c). To determine the distribution of specific glyco-receptors that might mediate the functions of this glycan-binding protein, Gal-1-binding sites were assessed by flow cytometry of mouse enterocytes using biotinylated rGal-1 (Figure 1d). Enterocytes displayed considerable expression of glyco-epitopes that bound exogenous rGal-1. A dose-response curve of lectin binding analyzed by flow cytometry demonstrated that as low as 15 ng/ml Gal-1 was sufficient to bind cell surface glycans on enterocytes (data not shown). Carbohydrate-dependent binding of rGal-1 was demonstrated by co-incubation with 100 mM lactose, (Figure 1d). To determine the glycophenotype of small intestinal tissue, we used a panel of plant lectins that bind to specific glycan structures and determine susceptibility or resistance to Gal-1 (Figure 1e). Consistent with the extensive binding of Gal-1 to enterocytes in the tip of the villi, these cells also bound peanut agglutinin (PNA), a lectin that binds to asialo-galactose-β1-3-N-acetylgalactosamine core-1 O-glycans and marks the absence of α2-3 sialylation of core-1 O-glycans, -phytohemagglutinin (PHA-L), a lectin that recognizes β1,6-N-acetylglucosamine-branched complex N-glycans and Lycopersicon esculentum (LEL), a lectin that recognizes polylactosamine-enriched glyco-epitopes.5 In line with this glyco-profile, we found no specific reactivity for the Sambucus nigra agglutinin (SNA), suggesting the absence of α2-6 sialylated-terminal glycans on epithelial cells. Collectively these results confirm the abundance of Gal-1 and Gal-1-binding sites within murine small intestine, suggesting a potential role for lectin-glycan lattices in modulating enterocyte homeostasis.
Gal-1 induces apoptotic death of mouse enterocytes
As Gal-1 has been reported to modulate the survival of distinct cell types, including T cells and tumor cells, through induction of apoptosis,4, 11, 18, 19 we examined the pro-apoptotic effects of this lectin on enterocytes isolated from mouse small bowel. Freshly isolated enterocytes were incubated in the presence of 10 μM rGal-1 for 2, 4 and 16 h and binding of annexin V was analyzed as a marker of early apoptosis (Figure 2a). Isolated enterocytes exposed to rGal-1 showed increased annexin V staining in a time-dependent manner. Although enterocytes spontaneously underwent apoptosis after being extracted from the mucosa (time=0) (Figure 2a), annexin V staining intensity was higher in cells incubated with rGal-1 as incubation time was prolonged, as compared with controls cultured in medium alone. A percentage of cells incubated with rGal-1 were annexin V− suggesting that a restricted population of epithelial cells were resistant to the pro-apoptotic effects of this lectin. The frequency of CD3+ T cells in cell extracts was below 3% in all cases as analyzed by flow cytometry (data not shown). Interestingly, upon incubation with rGal-1 phosphatidylserine exposure occurred in a concentration- and saccharide-dependent manner (Figure 2b). Apoptosis of enterocytes was evidenced by flow cytometry using the terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay (Figure 2c). Enterocytes treated with rGal-1 for 16 h showed an increased percentage of TUNEL-positive cells (51±3.5 versus 27.10±1 compared with cells cultured with medium alone; P< 0.01). Thus, exposure to Gal-1 engages an apoptotic program in mouse primary enterocytes.
Characterization of the Gal-1 apoptotic pathway in enterocytes
To further understand the mechanisms underlying Gal-1-induced cell death, isolated enterocytes were exposed to Gal-1 in the absence or presence of the ZVAD-FMK (benzyloxycarbonyl-Val-Ala-Asp (OMe) fluoromethylketone), a pan-caspase inhibitor (Figure 3a). Results showed a statistically significant inhibition of apoptosis induced by Gal-1 in enterocytes previously exposed to ZVAD-FMK, suggesting that Gal-1-induced cell death proceeds through a caspase-dependent pathway. As clearly seen, not all cells isolated from the epithelial compartment underwent apoptosis in the presence of rGal-1, which reflects the differential sensitivity of some sub-populations to the effects of this glycan-binding protein. To investigate whether caspase-3 is involved in this pathway, cells cultured with rGal-1 for 16 h were lysed and caspase-3 activity was assessed using the specific fluorometric substrate Ac-DEVD-AFC (N-acetyl-Asp-Glu-Val-Asp-AFC 7-amino-4-trifluoromethyl coumarin) (Figure 3b). When cells were exposed to rGal-1, fluorescent staining increased in a dose-dependent manner, an effect which was almost completely prevented by co-incubation of Gal-1 with a caspase-3-specific inhibitor (DEVD-CHO) (Figure 3b). Our results indicate that Gal-1 signals a caspase-3-dependent apoptotic pathway in enterocytes. To further evaluate upstream mediators involved in this apoptotic mechanism, we analyzed variations of the mitochondrial membrane potential in enterocytes exposed to rGal-1 for 16 h. A significant percentage of cells displayed reduced mitochondrial membrane potential (higher green fluorescent and lower red fluorescence) (Figure 3c), suggesting a role of the mitochondrial pathway in Gal-1-induced enterocyte apoptosis.
Exogenous Gal-1 promotes mouse enterocyte apoptosis in vivo
To directly assess the physiologic relevance of Gal-1-induced apoptosis in vivo, ligated loop assays were performed. Mice were treated with a duodenal intralumenal injection of rGal-1, saline or BSA as a control protein. After 3 h, anesthetized animals were killed, the small intestine was excised and enterocytes were isolated. A statistically significant induction of apoptosis was achieved with 20 μM rGal-1 (Figure 4a). However, when mice were treated with the same concentration of BSA or saline solution no binding of annexin V was detected (Figure 4a). Moreover, similar results were obtained using Lgals1−/− mice, suggesting lack of contribution of endogenous over exogenous Gal-1 in enterocyte viability. To determine whether phosphatidylserine exposure was accompanied by execution of a full apoptotic program, TUNEL assay was carried out on intestinal samples by inmunofluorescence (Figure 4b). Few apoptotic cells could be detected in loops filled with saline (1–2 nuclei per 5 villi) or with BSA (not shown). Although few apoptotic cells were observed within the lamina propria, most TUNEL-positive nuclei were detected in the epithelial cell compartment of loops filled with rGal-1. Notably, most of TUNEL-positive epithelial cells were located in the tip of the villi (showed by arrows). These results indicate that mouse enterocytes are sensitive to in vivo induction of apoptosis by Gal-1. Of note, the frequency of TUNEL-positive cells was similar between Lgals1−/− and wild-type mice filled with saline or rGal-1 in ligated loop assays (data not shown).
Endogenous Gal-1 controls mice villus length
It has been demonstrated that enterocytes located on the tip of the villus undergo apoptosis and shedding, and this effect may influence the length of the villus.20 We studied the potential role of endogenous Gal-1 in modulating mouse enterocyte apoptosis, and hence the villus length using duodenum from wild-type and Lgals1−/− mice. Samples were routinely fixed, stained with haematoxilin/eosin and visualized by optical microscopy. Although villus morphology was similar in wild-type and Lgals1−/− mice, villus from Gal-1-deficient mice was significantly longer (P< 0.002) (Figure 5a), indicating a potential role of endogenous Gal-1 in modulating epithelial cell survival. Of note, no significant histological differences were observed in other organs studied (liver, kidney, heart, thymus, lung, spleen) (data not shown). To rule out the possibility that longer villi in Lgals1−/− mice could be associated with an increased proliferative response in the crypts, we performed an in vivo assay with BrdU and the binding to FITC-labeled anti-BrdU was analyzed by flow cytometry. No statistical difference could be observed in the percentage of BrdU-positive cells between Lgals1−/− and wild-type mice (17.1±1.8 versus 16.7±0.6, respectively) (Figure 5b). When proliferation cell nuclear antigen (PCNA) was analyzed by immunohistochemistry, positive cells were observed in the crypt of all samples and no statistical difference was observed when comparing wild-type versus Lgals1−/− mice (10.16±2.11 versus 9.58±1.08 cells/crypt, respectively) (data not shown). Thus, endogenous Gal-1 regulates villus length through mechanisms involving modulation of cell death rather than proliferation of epithelial cells.
Starvation differentially modulates villus length in wild-type and Lgals1−/− mice
To further evaluate the pathophysiologic relevance of our findings, animals were starved for 48 h as this process conditions mouse enterocytes to engage apoptotic programs.21 Compared with fasted Lgals1−/− mice, fasted wild-type mice showed reduced villus length and a diminished small intestine weight in response to starvation (Figures 6a–c). However, no significant differences were observed in villi length and intestine weight between fasted and ad libitum fed Lgals1−/− mice. Enterocyte apoptosis was also evaluated using annexin V staining. The percentage of annexin V positive cells was significantly higher in wild-type fasted mice as compared with Lgals1−/− counterparts, consistent with reduced villus length and intestinal weight data (Figure 6d). In addition, we observed an increase in induction of Lgals1 transcript (1.25-fold increase) and Gal-1 protein (1.7-fold increase) in fasted versus ad libitum fed wild-type mice as determined by qRT-PCR (Figure 6e) and immunoblot analysis (data not shown). These results demonstrate that endogenous Gal-1 is required for epithelial cell survival and response to starvation.
Gal-1 induces apoptosis of human enterocytes
Given the potential biomedical implications of our findings, we then sought to examine the effect of Gal-1 in the induction of apoptosis of human yeyunum enterocytes. Surgical specimens of normal human small bowel were employed and enterocytes were detached from the mucosa and analyzed by fluorescence microscopy. Apoptosis was assessed by staining isolated cells with ethidium bromide and acrydine orange upon incubation with rGal-1 (Figure 7a). The basal percentages of apoptotic and necrotic cells were highly variable among samples (20±10% apoptotic cells and 10±8% necrotic cells). Notably, incubation of isolated enterocytes with rGal-1 for 16 h yielded a highly significant percentage of apoptotic cells (90±2%). When assessed by flow cytometry, Gal-1 induced apoptosis of human enterocytes in a dose-dependent manner (Figures 7b and c). These results indicate that human enterocytes are highly sensitive to the pro-apoptotic effect of this glycan-binding protein.
Discussion
The homeostasis of the intestinal epithelium requires tight control and balance of the different processes of proliferation, differentiation, migration and apoptosis. This coordination requires intervention of multiple synchronized pathways. The epithelial compartment of the gut encompasses a high cell turnover and the preservation of normal morphology and function relies on a delicate balance between cell proliferation and death.20 Several mechanisms have been proposed to operate in enterocyte maturation and apoptosis along the crypt-villus axis. In fact, there are two main compartments where apoptosis is physiologically relevant: at the crypt level, to remove excess or unwanted stem cells, and at the tip of the villus to limit their life span and control villus length.20
As protein-glycan interactions have recently emerged as critical mediators of intestinal cell physiology and pathology,22, 23 here we investigated the role of Gal-1 in the induction of apoptosis of intestinal epithelial cells, mainly in senescent epithelial cells located near the top of the villus.20 We provide evidence on the role of Gal-1-glycan lattices in human and mouse enterocyte apoptosis. Induction of apoptosis by this glycan-binding protein has been widely described in a variety of cell subsets.4, 5, 10 Susceptibility of T cells to Gal-1-induced cell death has been well established and characterized in normal and pathological conditions, and it has been proposed to be regulated via Fas-dependent or -independent pathways.5, 18, 19 B cell lymphoma cells,24 and non-immune cells such as Leydig cells,25 prostate cancer cells,26 breast cancer cells and trophoblast tumors,27 have also found to be targets of the pro-apoptotic effects of Gal-1. Regarding intestinal cells, human colonic Caco-2 cells have been proposed to be susceptible to Gal-1-induced cell death, although the precise mechanisms and physiologic relevance of these findings have not yet been characterized.28 In the present study we provide the first evidence showing a role for Gal-1 in modulating the survival of human and mouse enterocytes isolated from the small bowel; this mechanism could influence the maturation process of these cells during their migration from the crypt to the tip of the villus. In this regard, it appears that the glycosylation machinery generates a differential array of cell surface glycans along the crypt/villus axis, which makes these cells sensitive to Gal-1-induced cell death. The synthesis of these glycan structures occurs via a complex and highly regulated process involving glycosyltransferases and glycosidases.4 Mechanisms of Gal-1-mediated death have been shown to vary among different cell types.11, 18, 19, 25 Using in vitro assays, we demonstrated using isolated epithelial cells that apoptosis is dependent on caspase activation, through the intrinsic mitochondrial pathway. Paclik et al.28 reported a calpain-dependent, caspase-independent, induction of apoptosis by Gal-1 in the tumor Caco-2 cell line.29 These results could be relevant in the context of genes that control apoptosis during colorectal cancer.30
Interestingly, we found that Gal-1 induces apoptosis of a discrete population of enterocytes. TUNEL-positive cells were detected in the tip of the villi when apoptosis was induced in vivo following administration of rGal-1. Strikingly, we found that Lgals1−/− mice exhibited longer villi as compared with wild type mice, which did not appear to be related to differences in cell proliferation. Moreover, when mice were subjected to physiologic stress (fasting during 48 h), animals devoid of Gal-1 did not significantly afford reduction of villus length and small bowel weight, as compared with their wild-type counterpart.
The glycosylation profile of cell surface glycoproteins and glycolipids can differ considerably among different gut cell populations, when compared with similar cell subsets from other organs and tissues.31 This effect might be probably related to the particular tolerogenic microenvironment prevalent in the gut, which imposes a differential glyco-profile through enhanced activity of sialyltransferases.22, 31 In the present work, by using biotinylated lectins, we found that enterocytes display a glycosylation profile characterized by the absence of α2-3 sialylation of core-1 O-glycans (PNA+ cells), β1,6-branched complex N-glycans (L-PHA+ cells) and poly-N-acetyl-lactosamine sequences (LEL+ cells) as well as the absence of α2,6-linked terminal sialic acid (SNA− cells). Interestingly, this phenotype is consistent with a glycosylation signature permissive for Gal-1 binding and subsequent cell death, as it has been demonstrated for the T-cell compartment.5 However, this glycophenotype may differ in an inflammatory setting where the balance between cell proliferation and apoptosis is altered.7 Although different molecular pathways have been proposed,32, 33 the mechanisms underlying cell death during inflammatory processes still remain uncertain. In this regard, the influence of glycosylation and the regulatory effect of glycan-binding proteins have not been carefully studied in the epithelial cell compartment.
In this study, we showed that Gal-1 induces apoptosis both in vitro and in vivo, a process that was prevented by addition of the specific disaccharide lactose. This suggests that cell viability may be dependent on the glycosylation status of epithelial cell surface receptors and may be spatio-temporally regulated during the differentiation of these cells along the crypt-villus axis. Although basal levels of apoptosis were observed once epithelial cells underwent anoikis, a concentration- and time-dependent induction of cell death was observed in cells exposed in vivo (ligated loop assay) or in vitro to rGal-1.
Regarding the source of endogenous Gal-1, this lectin has been reported to be distributed within the whole mammalian digestive tract. Human gut expresses Gal-1 both in the epithelial compartment and in the lamina propria.13 In the mouse gut, this lectin is expressed diffusely in the lamina propria as well as in the muscle layer.34 However, the production of Gal-1 in the epithelial compartment is rather controversial.15, 34, 35 Mathieu et al.14 reported a differential distribution of this lectin that is highly dependent on the mouse strain analyzed. We found that intestinal cells belonging to mouse 129/SvJ strain express substantial amounts of Gal-1 and Gal-1-binding sites.
Remarkably, Gal-1 substantially decreased in TNBS-induced colitis, an effect that positively correlated with inappropriate mucosal T cell accumulation and resistance of lamina propria T cells to apoptosis,7 suggesting that Gal-1-glycan lattices may have a role in maintaining epithelial-immune homeostasis and integrity of intestinal mucosa. Our results indicate that Gal-1 serves as a key soluble factor involved in regulation of epithelial cell turnover and gut homeostasis. Lgals1−/− mice showed longer villi and wild-type mouse small intestine showed an altered response upon starvation. Overall, these results suggest that endogenous Gal-1 is implicated in the regulation of enterocyte viability under physiologic or stress situations. Remarkably, cell proliferation rate was found to be equivalent in wild-type and Lgals1−/− mice, suggesting that this process does not constitute a compensatory mechanism to restore the homeostatic environment.
In conclusion, our findings indicate for the first time that endogenous Gal-1 can execute an apoptotic program on human and mouse enterocytes, through activation of the mitochondrial pathway of cell death and this effect is more pronounced once the cell reaches the tip of the villus. This process may be critical in maintaining the integrity of the villus and sustaining the epithelial barrier function, thus favoring immune cell homeostasis and nutrient absorption. However, many questions still need to be addressed to further dissect the role of Gal-1-glycan interactions in different pathologic conditions of the gastrointestinal tract in which apoptosis contributes to disease pathogenesis, or is involved in the normal clearance of damaged cells (cancer, IBD, celiac disease or infectious diseases).32, 33, 36, 37
Materials and Methods
Intestinal epithelial cells
Cells were obtained from human and mouse small bowel. Wild-type and Lgals1−/− mice used in this study were of 129/SvJ background. Lgals1−/− mice were kindly provided by F Poirier (Institut Jacques Monod, Paris). Mice were bred at the animal facility of the Faculty of Exact Sciences, National University of La Plata according to institutional guidelines. The duodenum was washed, opened longitudinally and incubated for 10 min at 4 °C in buffer A (Hank's buffered saline solution (HBSS) supplemented with 100 IU/ml penicillin, 100 mg/ml streptomycin and 2% equine serum) with 1 mM dithiotreitol and then, with fresh buffer A plus 0.5 mM EDTA for 20 min at 37 °C with vigorous shaking. Cells were immediately resuspended in RPMI supplemented with 5% fetal bovine serum (FBS), penicillin/streptomycin and gentamicin. All protocols were approved by the Ethics Committee of the School of Exact Sciences, National University of La Plata.
Intestinal human resection specimens were obtained with the consent of patients undergoing gut transplantation at Fundación Favaloro (Buenos Aires, Argentina) according to Institutional Review Board guidelines for the use of human tissue. Tissue was collected onto ice-cold HBSS supplemented with penicillin/streptomycin until use. The mucosal layer was mechanically removed, washed with HBSS and incubated in HBSS/penicillin/streptomycin plus 0.5 mM EDTA. Cells were pelleted, resuspended in RPMI supplemented with 5% FBS, penicillin/streptomycin and used immediately.
Immunoblot analysis
Tissues were homogenized and cells were lysed as described previously.38 Samples were assayed for the presence of Gal-1 by using a 12% SDS-PAGE under reducing conditions (total protein load was 30 μg per lane) and immunoblotted with rabbit specific antiserum obtained as described before (dilution 1 : 3000), followed by horseradish peroxidase-conjugated goat anti-rabbit IgG. Membranes were stripped and probed for β-actin (rabbit anti-mouse β-actin antibody, diluted 1 : 4000, AbCam Cambridge, UK) as a loading control. Immunoblots were visualized with the ECL Plus System (GE Healthcare, Bucks, UK) according to manufacturer's instructions. The bands were scanned and quantified using the Adobe Photoshop CS software (version 8.0.1).
Real time RT-PCR
Total RNA extraction was performed using the NucleoSpin RNA II kit (Macherey-Nagel, Dueren, Germany). Reverse transcription was performed using random primers and MMLV-Reverse transcriptase (Invitrogen, Carlsbad, CA, USA). qPCR was performed following manufacturer's protocol using the iCycler thermal cycler (Bio-Rad, Hercules, CA, USA). Primer sequences for Gal-1 were used as described.6 Fold difference was represented by the ratio of the normalized value of each sample to that of mouse β-actin.39
Histology and immunohistochemistry
Mouse intestines were fixed in 5% neutral-buffered formalin and embedded in paraffin. Samples were cut and mounted onto positive-charged glass slides and heated at 60 °C for 30 min. Briefly, xylene and ethanol were used to de-paraffinize and endogenous peroxidase activity was blocked with 3% H2O2/methanol. Antigen retrieval was performed in buffer citrate (pH 6.0) and slides were microwaved. Non-specific sites were blocked with 1% BSA in PBS and incubated with primary and secondary antibodies. Counterstaining with 10% hematoxylin was performed and sections were mounted with Eukit. For routine histological examination, the length of duodenal villi was assessed using an ocular micrometer. The length of 10 well-oriented villi with both side-crypts visible was measured per cross-section, and the mean length was calculated. At least five sections per animal were analyzed.
PCNA was assessed as a nuclear marker of DNA synthesis and proliferation,40 using a specific anti-PCNA antibody (Santa Cruz, Santa Cruz, CA, USA), followed by an anti-rabbit IgG-biotin and strepavidin-HRP (Sigma, St. Louis, MO, USA). Immunostaining was then revealed using 3,3′-diaminobenzidine tetrahydrochloride (DAB) chromogen (DAKO, Glostrup, Denmark).
Lectin histochemistry was performed using biotinylated lectins as the primary binding reagent: SNA, PNA, Lycopersicon esculentum lectin (LEL) and Phaseolus vulgaris agglutinin (PHA) were all from Vector Labs (Edelberry; Burlingame, CA, USA) and used at a concentration of 25 μg/ml. Recombinant Gal-1 (rGal-1) (3 μg/ml) was biotinylated as described.5, 18 Streptavidin-HRP (DAKO) was then used as a secondary reagent and DAB was employed for the staining. Controls were performed by omitting the biotinylated lectins.
Immunohistochemistry for Gal-1 was carried out using a rabbit antiserum specific to Gal-1 obtained as described (dilution 1/3000). Primary antibody was incubated overnight at 4 °C and then goat anti-rabbit IgG conjugated to HRP (DAKO) was used for 1 h at 37 °C (dilution 1/3000). Finally, sections were incubated with DAB. Control with non-immune serum as primary antibody was performed.
TUNEL assay was performed essentially as described by the manufacturer (Promega, Madison, WI, USA). Nuclei were counterstained with DAPI and apoptotic cells were visualized by fluorescence microscopy.
Galectin binding assay
Gal-1 binding to enterocytes was performed by flow cytometry using decreasing concentrations of biotinylated Gal-1 ranging from 15 μg/ml–1.5 ng/ml followed by addition of streptavidin-APC (BD Pharmingen, Franklin Lakes, NJ, USA). Controls were included either by omitting the biotinylated lectin or by performing binding assays in the presence of 100 mM lactose.
Apoptosis assays
Freshly isolated enterocytes were incubated in RPMI 1640 medium supplemented with 5% FBS, penicillin/streptomycin at 37 °C in a humidified 5% CO2 atmosphere with different concentrations of rGal-1 during 2, 4 or 16 h. Saccharide-dependent binding of Gal-1 to enterocytes was assessed in the presence of 100 mM lactose as described.18 To analyze the mechanisms underlying epithelial cell death, cells were pre-treated with the pan-caspase inhibitor ZVAD-FMK (R&D Systems, Minneapolis, MN, USA). Controls included dimethyl sulfoxide (vehicle). As positive controls, cell death was induced with serum-deprived medium or with actinomycin D.
Apoptotic cells were also visualized by fluorescence microscopy.41 Cell suspension was mixed with an equal volume of a solution containing 0.01% ethidium bromide and 0.003% acridine orange. A Nikon Eclipse E400 microscope (Nikon, Tokyo, Japan) was used for visualization. Apoptosis was quantitatively analyzed by flow cytometry using double staining with annexin V-FITC/propidium iodide (PI). Briefly, cells were washed twice with PBS, and then incubated for 20 min with annexin V at 4 °C in the dark. Early (annexin V+/PI−) and late (annexin V+/PI+) apoptotic events were quantified. Fluorescence was detected using a FACSCalibur cytometer (BD Pharmigen).
TUNEL assay (Promega) was also used to study late apoptosis by flow cytometry according to manufacturer's instructions. Briefly, cells were washed, fixed with 4% paraformaldehyde in PBS, permeabilized with ethanol, and then incubated with TdT enzyme plus deoxinucleotides and fluorescein-12-dUTP for 1 h at 37 °C. Cells were washed, stained with PI and analyzed by flow cytometry (FACSCalibur cytometer). Fluorescein-12-dUTP incorporation results in green fluorescence within the nucleus of apoptotic cells; PI stains both apoptotic and non-apoptotic cells.
Fluorometric analysis of caspase-3 activity
Cells were lysed in ice-cold lysate buffer (10 mM EDTA, 50 mM Tris-HCl (pH 7.4), 150 mM sodium chloride, 1% Triton X100, 2 mM phenylmethylsulfonyl fluoride, 2 mM sodium orthovanadate, 10 mg/ml leupeptin and 2 mg/ml aprotinin) and the protein content was quantified with the bicinchoninic acid kit (Pierce BCA Protein Assay, Pierce, Rockford, IL, USA), using BSA as standard. Lysates were incubated with the caspase-3-specific substrate Ac-DEVD-AFC (Biomol International, Plymouth Meeting, PA, USA). Briefly, the fluorogenic AFC is released from the substrate by the caspase-3 action and it is spectrofluorometrically detected at 505 nm after excitation (400 nm). The arbitrary fluorescence units were normalized to the total protein content of the sample. In order to evaluate the specificity of the reaction, a control including the caspase-3 specific inhibitor Ac-DEVD-CHO (N-acetyl-Asp-Glu-Val-Asp-CHO aldehyde) (Biomol International) was performed.
Analysis of the mitochondrial pathway by flow cytometry
The Mitochondrial Permeability Detection Kit (MTt-E-ϕ, Biomol International) was used to analyze the mitochondrial membrane potential according to manufacturer's instructions. Briefly, 5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethyl-benzamidazolocarbocyanin iodide (JC-1) accumulates in healthy mitochondria giving rise to a red fluorescent signal. When the mitochondrial membrane potential declines as a consequence of the induction of apoptosis, a green fluorescence can be detected in mitochondria due to dye distribution within the cell.42, 19 Cells were incubated with the MTt-E-ϕ reagent for 15 min at 37 °C, and after washing they were resuspended in PBS. Fluorescence was detected by flow cytometry.
In vivo assessment of Gal-1-induced apoptosis in epithelial cells
Young male mice (N=5 per group), 25–30 g of weight, were fasted overnight and anaesthetized by intraperitoneal injection of ketamine (100 mg/kg) and xylacine (5 mg/kg). The ligated intestinal loop technique was performed as follows: a small longitudinal incision was practiced in the lower abdomen and a 2 cm section of small intestine from duodenum was clamped. Next, 100 μl of saline solution (control) or saline plus rGal-1 (20 μM) or BSA (20 μM) were injected in independent loops in the same animal. The abdominal wall was then sutured. During the procedure mice were kept warm on a 37 °C warming pad and anesthetized. Three hours later animals were killed by cervical dislocation and the different loops were individually processed. Apoptosis was evaluated by flow cytometry using annexin V/ PI staining and by TUNEL assay in paraffin-embedded tissue sections as previously described.
Proliferation assay
Proliferation was determined using bromodeoxyuridine (BrdU) In-Situ Detection Kit (BD Pharmingen) according to manufacturer's instructions. Briefly, wild-type and Lgals1−/− mice were given an intraperitoneal injection of 1 mg/ml BrdU in phosphate buffer (pH 7.4). Mice were killed 24 h later by cervical dislocation; enterocytes were isolated and further permeabilized. Cells were cultured with anti-BrdU labeled with FITC and fluorescence was analyzed by flow cytometry.
Starvation experiment
Adult wild-type (n=14) and Lgals1−/− (n=14) mice (weight 25–30 g) were used. All animals received water ad libitum. For each group seven animals were fed mouse chow ad libitum (control groups) whereas the remaining seven mice were starved for 48 h. Finally, all animals were killed and the small bowel was analyzed.
Statistical analysis
Comparison of two groups (means) was performed by using the one-way ANOVA and Student's t test for the comparison of paired samples. Results represent the mean±S.E.M. All results with P<0.05 were considered statistically significant.
Acknowledgments
We thank F Poirier (Institute Jacques Monod) for providing Lgals1−/− mice. We also thank Liliana Bruzzone for assistance with spectrofluorometric determinations, Carolina Zanuzzi for the histological sample preparation and Pablo Stringa for ligated loop assays. This work was supported by grants PICT 25417 (GHD) and PICT 2006-603 (GAR) from the Argentinean Agency for Promotion of Science and Technology, PIP 0938 (CM) from the Argentinean National Research Council (CONICET), University of Buenos Aires (GAR) and Fundación Sales (GAR).
Glossary
- Gal-1
galectin-1
- FBS
fetal bovine serum
- HBSS
Hank's buffered saline solution
- PCNA
proliferation cell nuclear antigen
- DAB
3,3′-diaminobenzidine tetrahydrochloride
- SNA
Sambucus nigra agglutinin
- PNA
peanut agglutinin
- LEL
Lycopersicon esculentum lectin
- PHA
Phaseolus vulgaris agglutinin
- ZVAD-FMK
benzyloxycarbonyl-Val-Ala-Asp (OMe) fluoromethylketone
- TUNEL
terminal deoxynucleotidyl transferase dUTP nick end labeling
- Ac-DEVD-AFC
N-acetyl-Asp-Glu-Val-Asp-AFC 7-amino-4-trifluoromethyl coumarin
- Ac-DEVD-CHO
N-acetyl-Asp-Glu-Val-Asp-CHO aldehyde
The authors declare no conflict of interest.
Footnotes
Edited by G Melino
References
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