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. 1992 Apr 11;20(7):1733–1737. doi: 10.1093/nar/20.7.1733

DnaG-dependent priming signals can substitute for the two essential DNA initiation signals in oriV of the broad host-range plasmid RSF1010.

Y Honda 1, T Nakamura 1, K Tanaka 1, A Higashi 1, H Sakai 1, T Komano 1, M Bagdasarian 1
PMCID: PMC312264  PMID: 1579467

Abstract

Broad host-range plasmid RSF1010 contains in the oriV region two DNA initiation signals, ssiA(RSF1010) and ssiB(RSF1010), which are essential for plasmid replication. Each of ssiA and ssiB could be substituted functionally by either of the two G4-type (DnaG-dependent) priming signals, the oric of bacteriophage G4 and an ssi signal from plasmid pSY343 (an R1 plasmid derivative). Functions of the chimeric oriVs of RSF1010 thus constructed were dependent on the RSF1010-specific replication proteins, RepA, RepB' and RepC. When both of ssiA and ssiB were replaced by the G4-type ssi signals, functions of the chimeric oriVs were no longer dependent on RepB' (RSF1010-specific DNA primase). The replication activities of the chimeric oriVs of RSF1010 were not influenced markedly by the type of heterologous priming signals they contained. It is conceivable that DNA replication of RSF1010 does not need the priming mechanism for lagging strand synthesis and proceeds by the strand displacement mechanism.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bahk J. D., Kioka N., Sakai H., Komano T. A runaway-replication plasmid pSY343 contains two ssi signals. Plasmid. 1988 Nov;20(3):266–270. doi: 10.1016/0147-619x(88)90033-9. [DOI] [PubMed] [Google Scholar]
  2. Bouché J. P., Rowen L., Kornberg A. The RNA primer synthesized by primase to initiate phage G4 DNA replication. J Biol Chem. 1978 Feb 10;253(3):765–769. [PubMed] [Google Scholar]
  3. Chung C. T., Niemela S. L., Miller R. H. One-step preparation of competent Escherichia coli: transformation and storage of bacterial cells in the same solution. Proc Natl Acad Sci U S A. 1989 Apr;86(7):2172–2175. doi: 10.1073/pnas.86.7.2172. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Drlica K., Rouviere-Yaniv J. Histonelike proteins of bacteria. Microbiol Rev. 1987 Sep;51(3):301–319. doi: 10.1128/mr.51.3.301-319.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Funnell B. E., Baker T. A., Kornberg A. In vitro assembly of a prepriming complex at the origin of the Escherichia coli chromosome. J Biol Chem. 1987 Jul 25;262(21):10327–10334. [PubMed] [Google Scholar]
  6. Godson G. N., Barrell B. G., Staden R., Fiddes J. C. Nucleotide sequence of bacteriophage G4 DNA. Nature. 1978 Nov 16;276(5685):236–247. doi: 10.1038/276236a0. [DOI] [PubMed] [Google Scholar]
  7. Grinter N. J., Barth P. T. Characterization of SmSu plasmids by restriction endonuclease cleavage and compatibility testing. J Bacteriol. 1976 Oct;128(1):394–400. doi: 10.1128/jb.128.1.394-400.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Haring V., Scholz P., Scherzinger E., Frey J., Derbyshire K., Hatfull G., Willetts N. S., Bagdasarian M. Protein RepC is involved in copy number control of the broad host range plasmid RSF1010. Proc Natl Acad Sci U S A. 1985 Sep;82(18):6090–6094. doi: 10.1073/pnas.82.18.6090. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Honda Y., Sakai H., Hiasa H., Tanaka K., Komano T., Bagdasarian M. Functional division and reconstruction of a plasmid replication origin: molecular dissection of the oriV of the broad-host-range plasmid RSF1010. Proc Natl Acad Sci U S A. 1991 Jan 1;88(1):179–183. doi: 10.1073/pnas.88.1.179. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Honda Y., Sakai H., Komano T., Bagdasarian M. RepB' is required in trans for the two single-strand DNA initiation signals in oriV of plasmid RSF1010. Gene. 1989 Aug 1;80(1):155–159. doi: 10.1016/0378-1119(89)90261-8. [DOI] [PubMed] [Google Scholar]
  11. Honda Y., Sakai H., Komano T. Two single-strand DNA initiation signals located in the oriV region of plasmid RSF1010. Gene. 1988 Sep 7;68(2):221–228. doi: 10.1016/0378-1119(88)90024-8. [DOI] [PubMed] [Google Scholar]
  12. Masai H., Arai K. Initiation of lagging-strand synthesis for pBR322 plasmid DNA replication in vitro is dependent on primosomal protein i encoded by dnaT. J Biol Chem. 1988 Oct 15;263(29):15016–15023. [PubMed] [Google Scholar]
  13. Masai H., Arai K. Leading strand synthesis of R1 plasmid replication in vitro is primed by primase alone at a specific site downstream of oriR. J Biol Chem. 1989 May 15;264(14):8082–8090. [PubMed] [Google Scholar]
  14. Masai H., Nomura N., Arai K. The ABC-primosome. A novel priming system employing dnaA, dnaB, dnaC, and primase on a hairpin containing a dnaA box sequence. J Biol Chem. 1990 Sep 5;265(25):15134–15144. [PubMed] [Google Scholar]
  15. Masai H., Nomura N., Kubota Y., Arai K. Roles of phi X174 type primosome- and G4 type primase-dependent primings in initiation of lagging and leading strand syntheses of DNA replication. J Biol Chem. 1990 Sep 5;265(25):15124–15133. [PubMed] [Google Scholar]
  16. Minden J. S., Marians K. J. Replication of pBR322 DNA in vitro with purified proteins. Requirement for topoisomerase I in the maintenance of template specificity. J Biol Chem. 1985 Aug 5;260(16):9316–9325. [PubMed] [Google Scholar]
  17. Nomura N., Low R. L., Ray D. S. Identification of ColE1 DNA sequences that direct single strand-to-double strand conversion by a phi X174 type mechanism. Proc Natl Acad Sci U S A. 1982 May;79(10):3153–3157. doi: 10.1073/pnas.79.10.3153. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Nomura N., Ray D. S. Expression of a DNA strand initiation sequence of ColE1 plasmid in a single-stranded DNA phage. Proc Natl Acad Sci U S A. 1980 Nov;77(11):6566–6570. doi: 10.1073/pnas.77.11.6566. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Nordström K., Molin S., Aagaard-Hansen H. Partitioning of plasmid R1 in Escherichia coli. II. Incompatibility properties of the partitioning system. Plasmid. 1980 Nov;4(3):332–339. doi: 10.1016/0147-619x(80)90071-2. [DOI] [PubMed] [Google Scholar]
  20. Scherzinger E., Bagdasarian M. M., Scholz P., Lurz R., Rückert B., Bagdasarian M. Replication of the broad host range plasmid RSF1010: requirement for three plasmid-encoded proteins. Proc Natl Acad Sci U S A. 1984 Feb;81(3):654–658. doi: 10.1073/pnas.81.3.654. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Scherzinger E., Haring V., Lurz R., Otto S. Plasmid RSF1010 DNA replication in vitro promoted by purified RSF1010 RepA, RepB and RepC proteins. Nucleic Acids Res. 1991 Mar 25;19(6):1203–1211. doi: 10.1093/nar/19.6.1203. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Schnos M., Zahn K., Inman R. B., Blattner F. R. Initiation protein induced helix destabilization at the lambda origin: a prepriming step in DNA replication. Cell. 1988 Feb 12;52(3):385–395. doi: 10.1016/s0092-8674(88)80031-x. [DOI] [PubMed] [Google Scholar]
  23. Scholz P., Haring V., Wittmann-Liebold B., Ashman K., Bagdasarian M., Scherzinger E. Complete nucleotide sequence and gene organization of the broad-host-range plasmid RSF1010. Gene. 1989 Feb 20;75(2):271–288. doi: 10.1016/0378-1119(89)90273-4. [DOI] [PubMed] [Google Scholar]
  24. Sims J., Capon D., Dressler D. dnaG (primase)-dependent origins of DNA replication. Nucleotide sequences of the negative strand initiation sites of bacteriophages St-1, phi K, and alpha 3. J Biol Chem. 1979 Dec 25;254(24):12615–12628. [PubMed] [Google Scholar]
  25. Uhlin B. E., Molin S., Gustafsson P., Nordström K. Plasmids with temperature-dependent copy number for amplification of cloned genes and their products. Gene. 1979 Jun;6(2):91–106. doi: 10.1016/0378-1119(79)90065-9. [DOI] [PubMed] [Google Scholar]
  26. Van der Ende A., Teertstra R., Weisbeek P. J. n' Protein activator sites of plasmid pBR322 are not essential for its DNA replication. J Mol Biol. 1983 Jul 5;167(3):751–756. doi: 10.1016/s0022-2836(83)80108-9. [DOI] [PubMed] [Google Scholar]
  27. Wada M., Kano Y., Ogawa T., Okazaki T., Imamoto F. Construction and characterization of the deletion mutant of hupA and hupB genes in Escherichia coli. J Mol Biol. 1988 Dec 5;204(3):581–591. doi: 10.1016/0022-2836(88)90357-9. [DOI] [PubMed] [Google Scholar]
  28. Yakobson E., Deiss C., Hirata K., Guiney D. G. Initiation of DNA synthesis in the transfer origin region of RK2 by the plasmid-encoded primase: detection using defective M13 phage. Plasmid. 1990 Jan;23(1):80–84. doi: 10.1016/0147-619x(90)90048-h. [DOI] [PubMed] [Google Scholar]
  29. Zipursky S. L., Marians K. J. Escherichia coli factor Y sites of plasmid pBR322 can function as origins of DNA replication. Proc Natl Acad Sci U S A. 1981 Oct;78(10):6111–6115. doi: 10.1073/pnas.78.10.6111. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. de Graaff J., Crosa J. H., Heffron F., Falkow S. Replication of the nonconjugative plasmid RSF1010 in Escherichia coli K-12. J Bacteriol. 1978 Jun;134(3):1117–1122. doi: 10.1128/jb.134.3.1117-1122.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]

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