Table 2.

Efficiency of the HBoV2 real-time PCR assay

Estimated pGEM-T NP-1 HBoV2 plasmid DNA copy no.	Mean CT ± SDa
101	41.32 ± 0.80
102	37.62 ± 0.37
103	35.06 ± 0.07
104	32.47 ± 0.07
105	30.13 ± 0.11
106	26.03 ± 0.10
107	21.69 ± 0.06
108	17.18 ± 0.04

^aThe values shown are the means of triplicate samples. The slope, −3.31, was determined from the formula y = y intercept − slope log₁₀. The slope was calculated from 10¹ to 10⁸ copies per reaction. The percent efficiency was 100.5. Efficiency = 10^(−1/slope) − 1.