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. 1992 Apr 25;20(8):1897–1901. doi: 10.1093/nar/20.8.1897

Construction of a chromosome-enriched HpaII library from flow-sorted wheat chromosomes.

M L Wang 1, A R Leitch 1, T Schwarzacher 1, J S Heslop-Harrison 1, G Moore 1
PMCID: PMC312304  PMID: 1374560

Abstract

We report here the first successful generation of a chromosome-enriched library from flow sorted plant chromosomes. Chromosomes with a characteristic DNA content (a peak) were sorted from a synchronized cell culture (TaKB1, derived from Triticum aestivum). A HpaII library was constructed from the sorted chromosomes and half of the cloned DNA sequences analysed are unique or low copy. Approximately half of these sequences when used as probes detect sequences on wheat chromosome 4A. The generation and analysis of the chromosome library is described in detail and the prospects of using flow-sorted plant chromosomes discussed.

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Selected References

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  1. Bennett M. D., Smith J. B. Nuclear dna amounts in angiosperms. Philos Trans R Soc Lond B Biol Sci. 1976 May 27;274(933):227–274. doi: 10.1098/rstb.1976.0044. [DOI] [PubMed] [Google Scholar]
  2. Clarke B., Stancombe P., Money T., Foote T., Moore G. Targeting deletion (homoeologous chromosome pairing locus) or addition line single copy sequences from cereal genomes. Nucleic Acids Res. 1992 Mar 25;20(6):1289–1292. doi: 10.1093/nar/20.6.1289. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Davies K. E., Young B. D., Elles R. G., Hill M. E., Williamson R. Cloning of a representative genomic library of the human X chromosome after sorting by flow cytometry. Nature. 1981 Oct 1;293(5831):374–376. doi: 10.1038/293374a0. [DOI] [PubMed] [Google Scholar]
  4. Flavell R. B. Repetitive DNA and chromosome evolution in plants. Philos Trans R Soc Lond B Biol Sci. 1986 Jan 29;312(1154):227–242. doi: 10.1098/rstb.1986.0004. [DOI] [PubMed] [Google Scholar]
  5. Frearson E. M., Power J. B., Cocking E. C. The isolation, culture and regeneration of Petunia leaf protoplasts. Dev Biol. 1973 Jul;33(1):130–137. doi: 10.1016/0012-1606(73)90169-3. [DOI] [PubMed] [Google Scholar]
  6. Gray J. W., Carrano A. V., Steinmetz L. L., Van Dilla M. A., Moore D. H., 2nd, Mayall B. H., Mendelsohn M. L. Chromosome measurement and sorting by flow systems. Proc Natl Acad Sci U S A. 1975 Apr;72(4):1231–1234. doi: 10.1073/pnas.72.4.1231. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kendall T. L., Byerley D. J., Dean R. Isolation of DNA from blood. Anal Biochem. 1991 May 15;195(1):74–76. doi: 10.1016/0003-2697(91)90297-7. [DOI] [PubMed] [Google Scholar]
  8. Russell G. J., Follett E. A., Subak-Sharpe J. H., Harrison B. D. The double-stranded DNA of cauliflower mosaic virus. J Gen Virol. 1971 Jun;11(3):129–138. doi: 10.1099/0022-1317-11-3-129. [DOI] [PubMed] [Google Scholar]
  9. SWARTZ M. N., TRAUTNER T. A., KORNBERG A. Enzymatic synthesis of deoxyribonucleic acid. XI. Further studies on nearest neighbor base sequences in deoxyribonucleic acids. J Biol Chem. 1962 Jun;237:1961–1967. [PubMed] [Google Scholar]

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