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. 1992 May 11;20(9):2271–2278. doi: 10.1093/nar/20.9.2271

The swi4+ gene of Schizosaccharomyces pombe encodes a homologue of mismatch repair enzymes.

O Fleck 1, H Michael 1, L Heim 1
PMCID: PMC312341  PMID: 1317550

Abstract

The swi4+ gene of Schizosaccharomyces pombe is involved in termination of copy-synthesis during mating-type switching. The gene was cloned by functional complementation of a swi4 mutant transformed with a genomic library. Determination of the nucleotide sequence revealed an open reading frame of 2979 nucleotides which is interrupted by a 68 bp long intron. The putative Swi4 protein shows homology to Duc-1 (human), Rep-3 (mouse), HexA (Streptococcus pneumoniae) and MutS (Salmonella typhimurium). The prokaryotic proteins are known as essential components involved in mismatch repair. A strain with a disrupted swi4+ gene was constructed and analysed with respect to the switching process. As in swi4 mutants duplications occur in the mating-type region of the swi4 (null) strain, reducing the efficiency of switching.

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Selected References

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